Saltar al contenido
MilliporeSigma

In vitro activities of amphotericin B deoxycholate and liposomal amphotericin B against 604 clinical yeast isolates.

Journal of medical microbiology (2014-09-12)
Maria Teresa Montagna, Grazia Lovero, Caterina Coretti, Osvalda De Giglio, Domenico Martinelli, Andrea Bedini, Mario Delia, Antonio Rosato, Mauro Codeluppi, Giuseppina Caggiano
RESUMEN

We determined the in vitro antifungal activity of liposomal amphotericin B (L-AmB) against 604 clinical yeast isolates. Amphotericin B deoxycholate (D-AmB) was tested in parallel against all the isolates. Susceptibility testing was performed according to the Clinical and Laboratory Standards Institute (CLSI) M27-A3 method. Overall, L-AmB was highly active against the isolates (mean MIC, 0.42 µg ml(-1); MIC90, 1 µg ml(-1); 97.2 % of MICs were ≤1 µg ml(-1)) and comparable to D-AmB (mean MIC, 0.48 µg ml(-1); MIC90, 1 µg ml(-1); 97.3 % of MICs were ≤1 µg ml(-1)). The in vitro activity of D-AmB and L-AmB was correlated (R(2) = 0.61; exp(b), 2.3; 95 % CI, 2.19-2.44, P<0.001). Candida albicans (mean MICs of D-AmB and L-AmB, 0.39 µg ml(-1) and 0.31 µg ml(-1), respectively) and Candida parapsilosis (mean MICs of D-AmB and L-AmB, 0.38 µg ml(-1) and 0.35 µg ml(-1), respectively) were the species most susceptible to the agents tested, while Candida krusei (currently named Issatchenkia orientalis) (mean MICs of D-AmB and L-AmB, 1.27 µg ml(-1) and 1.13 µg ml(-1), respectively) was the least susceptible. The excellent in vitro activity of L-AmB may have important implications for empirical treatment approaches and support its role in treatment of a wide range of invasive infections due to yeasts.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
D-(+)-Glucosa, ≥99.5% (GC)
Sigma-Aldrich
D-(+)-Glucosa, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
Sigma-Aldrich
Anfotericina B solution, 250 μg/mL in deionized water, 0.1 μm filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
MOPS, ≥99.5% (titration)
Sigma-Aldrich
Sodium deoxycholate, BioXtra, ≥98.0% (dry matter, NT)
Sigma-Aldrich
Dextrosa, 97.5-102.0% anhydrous basis, meets EP, BP, JP, USP testing specifications
Sigma-Aldrich
Anfotericina B from Streptomyces sp., powder, γ-irradiated, BioXtra, suitable for cell culture
Sigma-Aldrich
D-(+)-Glucosa, ≥99.5% (GC), BioXtra
Sigma-Aldrich
MOPS, BioPerformance Certified, suitable for cell culture, ≥99.5% (titration)
USP
D-(+)-Glucosa, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Anfotericina B from Streptomyces sp., ~80% (HPLC), powder
Sigma-Aldrich
D-(+)-Glucosa, BioUltra, anhydrous, ≥99.5% (sum of enantiomers, HPLC)
Sigma-Aldrich
MOPS, BioXtra, ≥99.5% (titration)
SAFC
Sodium deoxycholate
Supelco
D-(+)-Glucosa, analytical standard
Sigma-Aldrich
Deoxycholic acid, ≥98% (HPLC)
Sigma-Aldrich
Anfotericina B from Streptomyces sp., BioReagent, suitable for cell culture, ~80% (HPLC)
Sigma-Aldrich
D-(+)-Glucosa, suitable for mouse embryo cell culture, ≥99.5% (GC)
Sigma-Aldrich
Deoxycholic acid, ≥99.0% (T)
Sigma-Aldrich
Sodium deoxycholate, ≥97% (titration)
Sigma-Aldrich
D-(+)-Glucosa, Hybri-Max, powder, BioReagent, suitable for hybridoma
Sigma-Aldrich
D-(+)-Glucosa, ACS reagent
Supelco
D-(+)-Glucosa, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
D-(+)-Glucosa, 99.9 atom % 16O, 99.9 atom % 12C
Sigma-Aldrich
MOPS, anhydrous, free-flowing, Redi-Dri, ≥99.5%
Sigma-Aldrich
MOPS, BioUltra, for molecular biology, ≥99.5% (titration)
Amphotericin B, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
D-(+)-Glucosa, tested according to Ph. Eur.
Amphotericin B for microbiological assay, European Pharmacopoeia (EP) Reference Standard
Amphotericin B for peak identification, European Pharmacopoeia (EP) Reference Standard