W2394

Anti-WIPI-1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinónimos: Anti-ATG18, Anti-WD repeat domain phosphoinositide interacting 1, Anti-WD40 repeat protein Interacting with phosphoInositides of 49kDa, Anti-WIPI-1 alpha, Anti-WIPI49
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• conjugado: unconjugated
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~49 kDa
• reactividad de especies: human, rat, mouse
• concentración: ~1.0 mg/mL
• técnicas: immunoprecipitation (IP): 5-10 μg using lysates of mouse 3T3 or at NRK cells; western blot: 2.5-5.0 μg/mL using whole extract of G-361 cells
• Nº de acceso UniProt: Q5MNZ9
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... WIPI1(55062) ; mouse ... Wipi1(52639) ; rat ... Wipi1(303630)

Descripción

Descripción general

WIPI-1, the mammalian orthologue of Atg18 in S. cerevisiae and A. thaliana, is a member of the WIPI subfamily of WD-repeat proteins. It has a 7-bladed propeller structure and contains a conserved motif for interaction with phospholipids. WIPI-1 is ubiquitously expressed in normal human tissues with highest levels in skeletal muscle, heart and testis. It is aberrantly expressed in human cancer.

Inmunógeno

synthetic peptide corresponding to amino acids 406-421 of human WIPI-1, conjugated to KLH. The corresponding sequence is identical in mouse and rat.

Aplicación

Anti-WIPI-1 (C-terminal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Acciones bioquímicas o fisiológicas

WIPI-1 has essential biological functions including signal transduction, transcription regulation and apoptosis. WD-repeat proteins regulate the assembly of multiprotein complexes by presenting a β-propeller platform for simultaneous and reversible protein-protein interactions. WIPI-1 is linked to starvation-induced autophagy.

Forma física

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)