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V5507

Sigma-Aldrich

Monoclonal Anti-VSV Glycoprotein antibody produced in mouse

clone P5D4, ascites fluid

Sinónimos:

Monoclonal Anti-VSV Glycoprotein

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100 μL
$496.00
0.2 ML
$647.00

$496.00


Fecha estimada de envío28 de mayo de 2025


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100 μL
$496.00
0.2 ML
$647.00

About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

$496.00


Fecha estimada de envío28 de mayo de 2025


Solicitar un pedido a granel

origen biológico

mouse

conjugado

unconjugated

forma del anticuerpo

ascites fluid

tipo de anticuerpo

primary antibodies

clon

P5D4, monoclonal

contiene

15 mM sodium azide

técnicas

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1:100,000 using whole cell extracts expressing VSV-G tagged fusion protein

isotipo

IgG1

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

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Especificidad

The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain. This antibody has been used for studies on the role of the cytoplasmic domain on newly-synthesized VSV-G during transfer to the plasma membrane and cell surface, using micro-injected antibody, immunoblotting, immunoprecipitation, immunocytochemistry and immunoelectron microscopy. The antibody has been used for the detection, immunoprecipitation and immunocytochemical staining of exogenously introduced constructs tagged with the carboxyl-terminus of VSV-G. This tag does not interfere with the function of the studied protein and can be specifically recognized by the P5D4 antibody without cross-reaction with any endogenous protein.

Inmunógeno

synthetic peptide containing the 15 carboxy-terminal amino acids (497-511) of Vesicular Stomatitis Virus Glycoprotein (VSV-G), conjugated to KLH.

Aplicación

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)

Almacenamiento y estabilidad

For continuous use, store at 2 °C to 8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Jonathan Chow et al.
Cell host & microbe, 22(1), 48-60 (2017-07-14)
Asymptomatic infections often proceed undetected, yet can still prime the host to be sensitive to secondary environmental stress. While the mechanisms underlying disease caused by asymptomatic infections are unknown, it is believed that productive pathogen replication is required. We report
Xiuran Niu et al.
Cell death & disease, 9(10), 971-971 (2018-09-27)
Chaperone-assisted proteasome degradation of oncogenic protein acts as an upstream signal controlling tumorigenesis and progression. The understanding of the co-regulation of chaperone and oncoprotein of endocytosis pathways is extremely limited. In this study, we showed for the first time that
Eric L Haseltine et al.
Bulletin of mathematical biology, 70(6), 1730-1748 (2008-04-26)
Although many tools of cellular and molecular biology have been used to characterize single intracellular cycles of virus growth, few culture methods exist to study the dynamics of spatially spreading viruses over multiple generations. We have previously developed a method
Samartha G Anekal et al.
Integrative biology : quantitative biosciences from nano to macro, 1(11-12), 664-671 (2009-12-23)
The dynamics of viral infection spread, whether in laboratory cultures or in naturally infected hosts, reflects a coupling of biological and physical processes that remain to be fully elucidated. Biological processes include the kinetics of virus growth in infected cells
Ying Zhu et al.
Biomedical microdevices, 11(3), 565-570 (2009-01-15)
The plaque assay has long served as the "gold standard" to measure virus infectivity and test antiviral drugs, but the assay is labor-intensive, lacks sensitivity, uses excessive reagents, and is hard to automate. Recent modification of the assay to exploit

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