Products with a high water content away from "the generally known reaction partners of water." This caution is due to water's potential to react violently with numerous substances, resulting in the development of gases and the ignition of released Hydrogen, posing a risk of explosion.
Additionally, various substances or mixtures can explode upon the addition of a drop of water, with details of these reactions provided in the safety data sheets of the specific reaction partners. Substance groups that react violently with water include Alkaline earth metals, Alkaline metals, Aluminum alkalines, Metallic or Nonmetallic hydrides, Metallic or Nonmetallic oxides, Acid anhydrides, and Acid chlorides.
V5265
Crystal Violet Solution
1%, aqueous solution
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$82.60
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250 ML
$82.60
500 ML
$91.80
About This Item
Fórmula empírica (notación de Hill):
C25H30ClN3
Número de CAS:
Peso molecular:
407.98
Beilstein:
3580948
Número MDL:
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
NACRES:
NA.47
Formulario
aqueous solution
concentración
1%
técnicas
microbe id | staining: suitable
color
deep violet, purple
Amáx.
0.36-0.44 at 589-594 nm
aplicaciones
diagnostic assay manufacturing
hematology
histology
temp. de almacenamiento
room temp
cadena SMILES
[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C
InChI
1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
Clave InChI
ZXJXZNDDNMQXFV-UHFFFAOYSA-M
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Descripción general
Crystal violet, also known as gentian violet or methyl violet, is the N-hexamethylated derivative of pararosaniline. It is a basic, cationic, acidotropic protein dye used in histological and bacteriological staining. It is well-known for its antibacterial and antifungal properties.[1]
It stains the fatty portions of sebaceous sweat a deep purple color. Crystal violet can also be used to enhance bloody fingerprints. This dye is harmful if inhaled, swallowed or absorbed through skin, contact may cause cancer, severe eye irritation in human beings.[2]
It stains the fatty portions of sebaceous sweat a deep purple color. Crystal violet can also be used to enhance bloody fingerprints. This dye is harmful if inhaled, swallowed or absorbed through skin, contact may cause cancer, severe eye irritation in human beings.[2]
Aplicación
- Crystal violet is commonly used in Gram staining for the classification of bacteria.[3]
- It has also been used to detect bacterial adherence to biomedical polymers and to stain DNA in mammalian tissues in Giemsa staining.
- It has been successfully used to develop a counterion-staining method to detect DNA in agarose gel electrophoresis.[4]
- Its antimicrobial properties have facilitated its use in the treatment of oral candidiasis, skin infections, and methicillin-resistant Staphylococcus aureus.[1]
- It has been used as a stain in cell proliferation assays, migration assays, and Boyden chamber assay.[5][6][7]
Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining.[8]
Acciones bioquímicas o fisiológicas
The antimicrobial effects of crystal violet can be attributed to free radical generation and formation of unionized bacterial complexes with the dye. It has also been reported to inhibit protein synthesis and bacterial cell wall formation. It is especially effective against Gram-positive bacteria.[1]
Principio
Crystal violet is the primary stain in Gram staining. It infiltrates the peptidoglycan-rich cell wall of Gram-positive bacteria, and forms entrapped complexes with Gram′s iodine that retain the purple color post-decolorization. Thus, Gram-positive bacteria can be differentiated from Gram-negative bacteria whose peptidoglycan layer is considerably thinner.
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Certificados de análisis (COA)
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Characterization of potent and selective iodonium-class inhibitors of NADPH oxidases
Lu J, et al.
Biochemical Pharmacology, 43, 25-38 (2017)
K Engbaek et al.
Journal of clinical pathology, 32(2), 187-190 (1979-02-01)
Five techniques for Gram staining bacteria in paraffin sections were compared on serial sections of pulmonary tissues from eight bacteriological necropsies. Brown and Hopp's method was the most satisfactory for distinguishing Gram-positive and Gram-negative bacteria. However, this method cannot be
Histological and histochemical methods: Theory and practice (5th ed.)
JA Kiernan
Histological and Histochemical Methods: Theory and Practice (2015)
Studies on adsorption of crystal violet dye from aqueous solution onto coniferous pinus bark powder (CPBP)
Ahmad R.
Journal of Hazardous Materials, 171, 767-773 (2009)
Gentian Violet: a 19th century drug re-emerges in the 21st century
Alexander M. Maley, Jack L. Arbiser
Experimental Dermatology, 22(12) (2013)
Contenido relacionado
Visión de conjunto de la ciencia y la práctica de la bacteriología en el diagnóstico clínico. Más información sobre la aplicación de colorantes convencionales y especiales para el análisis microscópico.
An overview of the science and practice of bacteriology in clinical diagnostics. Learn more about the application of standard and special stains for microscopic analysis.
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What does "generally known reaction partners of water" refer to in section 10.3 of the V5265 SDS? Additionally, in the SDS for a similar product, C6158, states that violent reactions are possible with strong oxidizing agents, strong acids, and oxidizing agents.
1 answer-
Helpful?
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What solvent is the crystal violet diluted in? Can it be used for staining cells for colony formation assay directly or will fixation with methanol/ethanol be needed prior to the staining?
1 answer-
This product is a 1% solution of Crystal Violet in water. For colony formation assays, it is more common to dissolve the crystal violet in a methanol-based solution. However, it is possible to fix the cells in methanol for 10-15 minutes prior to staining cells with an aqueous Crystal Violet Solution. Some sources recommend substituting absolute ethanol for methanol. Following fixation, the cells can be stained with aqueous Crystal Violet solution.
Helpful?
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What is the shelf life / expiry of this solution?
1 answer-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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