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TOX7

Sigma-Aldrich

In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based

Sinónimos:

LDH cytotoxicity assay

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.84

form

liquid

usage

 kit sufficient for 500 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

protein quantification: suitable

λmax

490 nm

application(s)

cell analysis
detection

detection method

colorimetric

Storage temp.

−20°C

Categorías relacionadas

General description

Tox7 is a member of Sigma′s CytoToxicity Kit series. It is based on the measurement of lactate dehydrogenase (LDH) release from cells as a measure of cell membrane damage and cytotoxicity. This technique has been utilized as an alternative to 51Cr release for cell mediated cytotoxicity assays, as well as conventional cytotoxicity resulting from interaction of a test material with the cell.

Application

In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based has been used:
  • to detect lactate dehydrogenase release in human umbilical vein endothelial cells (HUVECs) and human cardiac myocytes (HCMs) for lactate dehydrogenase (LDH) assay
  • to determine the activity of lactate dehydrogenase leakage of MDA-MB-231 cells
  • to examine the activity of LDH in RAW 264.7 cells , in LDH cytotoxicity assay in human cell lines
  • for spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 490 nm.

Biochem/physiol Actions

The lactate dehydrogenase assay is a means of measuring either the number of cells via total cytoplasmic lactate dehydrogenase (LDH) or membrane integrity as a function of the amount of cytoplasmic LDH released into the medium. This method is simple, accurate and yields reproducible results. The assay is based on the reduction of NAD by LDH. The resulting reduced NAD (NADH) is utilized in the stoichiometric conversion of a tetrazolium dye to a soluble, colored formazan derivative.
.

hcodes

H412

Hazard Classifications

Aquatic Chronic 3

Storage Class

10 - Combustible liquids

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Tryptophan metabolites: kynurenine (KYN), kynurenic acid (KYNA) and 6-formylindolo[3,2-b]carbazole (FICZ) are considered aryl hydrocarbon receptor (AhR) ligands. AhR is mainly expressed in barrier tissues, including skin, and is involved in various physiological and pathological processes in skin. We studied the
Effects of 5-fluorouracil on morphology, cell cycle, proliferation, apoptosis, autophagy and ROS production in endothelial cells and cardiomyocytes
Focaccetti C, et al.
Testing, 10, e0115686-e0115686 (2015)
Christian Polley et al.
Materials (Basel, Switzerland), 13(7) (2020-04-15)
The prevalence of large bone defects is still a major problem in surgical clinics. It is, thus, not a surprise that bone-related research, especially in the field of bone tissue engineering, is a major issue in medical research. Researchers worldwide
Cytotoxicity of biologically synthesized silver nanoparticles in MDA-MB-231 human breast cancer cells
Gurunathan S, et al.
BioMed Research International (2013)
Riboflavin deprivation inhibits macrophage viability and activity-a study on the RAW 264.7 cell line
Mazur-BAI, et al.
The British Journal of Nutrition, 110(3), 509-514 (2013)
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