T9157
Tryptose Phosphate Broth
suitable for insect cell culture
Sinónimos:
Tryptose Broth
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100 G
$35.82
500 G
$169.00
$35.82
Precio de catálogo$48.40Ahorre 26 %Web-Only Promotion
Disponible para envío el08 de abril de 2025Detalles
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100 G
$35.82
500 G
$169.00
About This Item
Código UNSPSC:
41106507
NACRES:
NA.73
$35.82
Precio de catálogo$48.40Ahorre 26 %Web-Only Promotion
Disponible para envío el08 de abril de 2025Detalles
Solicitar un pedido a granel
Productos recomendados
origen biológico
bovine milk
Formulario
solid
técnicas
cell culture | insect: suitable
Condiciones de envío
ambient
temp. de almacenamiento
room temp
Descripción general
Tryptose Phosphate Broth (TPB) comprises pyrimidine based components and is crucial for pyrimidine synthesis and electron transport chain in cells. In chick embryo fibroblast, it alleviates chloramphenicol inhibition.[1]
Aplicación
In addition to its use for the growth of fastidious micro-organisms, Tryptose Phospate Broth (TPB) has been studied as supplement for the preparation of media that supports vaccine production in BHK-21 cells and the growth of SF21 insect cells in high-density perfusion culture stirred-tank bioreactors.
Tryptose Phosphate Broth has been used as a supplement:
- in Eagle′s minimum essential medium alpha for growing Vero cells[2]
- for Vero-CCL81 cells in porcine epidemic diarrhea coronavirus propagation[3]
- in Dulbecco modified Eagle medium in LR7cells for mouse hepatitis virus propagation[4]
- in Glasgow minimum essential medium (GMEM) for Madin-Darby canine kidney (MDCK) cells.[5]
Componentes
Tryptose Phosphate Broth (TPB) is composed of four components: Tryptose (20g/L); Dextrose (2g/L); NaCl (5g/L) and Disodium Phosphate (2.5g/L) typically adjusted to pH 7.3. The tryptose component is a peptone (mixture of amino acids and short peptides) derived by the mixed enzymatic hydrolysis (pancreatic enzymes) of the milk protein casein. This hydrolysate provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious mico-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity.
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Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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Identification and characterization of a proteolytically primed form of the murine coronavirus spike proteins after fusion with the target cell
Wicht O, et al.
Journal of Virology, 88(9), 4943-4952 (2014)
Azali Azlan et al.
PLoS neglected tropical diseases, 15(1), e0008351-e0008351 (2021-01-23)
The Asian tiger mosquito, Aedes albopictus (Ae. albopictus), is an important vector that transmits arboviruses such as dengue (DENV), Zika (ZIKV) and Chikungunya virus (CHIKV). Long noncoding RNAs (lncRNAs) are known to regulate various biological processes. Knowledge on Ae. albopictus
Aminopeptidase N is not required for porcine epidemic diarrhea virus cell entry
Li W, et al.
Virus Research, 235, 6-13 (2017)
Tryptose phosphate broth improves Rickettsia felis replication in mammalian cells
Saisongkorh W, et al.
FEMS Immunology and Medical Microbiology, 64(1), 111-114 (2012)
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