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T4661

Sigma-Aldrich

Base Trizma®

≥99.9% (titration), crystalline

Sinónimos:

2-Amino-2-(hidroximetil)-1,3-propanodiol, Base tris, THAM, Tris(hidroximetil)-aminometano, Trometamol

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100 G
$72.20
1 KG
$318.00
5 KG
$1,480.00

$72.20

Disponible para envío el10 de abril de 2025Detalles

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100 G
$72.20
1 KG
$318.00
5 KG
$1,480.00

About This Item

Fórmula lineal:
NH2C(CH2OH)3
Número de CAS:
77-86-1
Peso molecular:
121.14
Beilstein:
741883
Número CE:
201-064-4
Número MDL:
MFCD00004679
Código UNSPSC:
12352104
ID de la sustancia en PubChem:
24900061
NACRES:
NA.25

$72.20

Disponible para envío el10 de abril de 2025Detalles

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descripción

aminopeptidase substrate

Nivel de calidad

400

Ensayo

≥99.9% (titration)

Formulario

crystalline

pérdida

≤5% (loss on drying, 110 °C)

color

white

pH

10.0-11.5

intervalo de pH útil

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubilidad

water: soluble (400 g plus 600 mL of water)

λ

(290nm < 0.05)
 in 5 mM Tris; DNA (40% (w/w))

aplicaciones

diagnostic assay manufacturing

cadena SMILES

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

Clave InChI

LENZDBCJOHFCAS-UHFFFAOYSA-N

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Descripción general

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology.[1] It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc.[2] It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.[3]

Aplicación

Tris base has been used:
  • As a component of lysis buffer for cell disruption[4][5]
  • In the preparation of TBE solution for PAGE (polyacrylamide gel electrophoresis)[6][5]
  • In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC[7]
  • In capillary electrochromatography and UV analysis of tocopherols and tocotrienols[8]

Envase

Meets all specifications for T1503. For biotechnology, diagnostics or manufacturing use. Please inquire for commercial quantities.

Nota de análisis

Not pyrogen tested. Certificates of analysis are available with lot specific information.

Otras notas

Los valores de pH de todas las disoluciones tampón son dependientes de la temperatura y la concentración. Para los tampones Tris, el pH aumenta alrededor de 0,03 unidades por °C de disminución de la temperatura, y disminuye 0,03-0,05 unidades al ser diluido por 10.
Para aplicaciones precisas, debe utilizarse un peachímetro cuidadosamente calibrado con un electrodo de vidrio/calomelano combinados.

Información legal

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
0000430883
0000430883
0000419003
0000419021
0000419022

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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Enzymatic Activity Assays for Base Excision Repair Enzymes in Cell Extracts from Vertebrate Cells.
Caglayan M, et al.
Bio-protocol, 5(11) (2015)
Accumulated lipids rather than the rigid cell walls impede the extraction of genetic materials for effective colony PCRs in Chlorella vulgaris.
Tear CJY, et al.
Microbial cell factories, 12(1), 106-106 (2013)
Genome-wide mapping of embedded ribonucleotides and other noncanonical nucleotides using emRiboSeq and EndoSeq
Ding J, et al.
Nature Protocols, 10(9), 1433-1433 (2015)
Recognition and strand displacement of DNA oligonucleotides by peptide nucleic acids (PNAs). High-performance ionexchange chromatographic analysis
Lesignoli F, et al.
Journal of Chromatography A, 922(1-2), 177-185 (2001)
Capillary electrochromatographic evaluation of vitamin active oil constituents: tocopherols and tocotrienols.
Abidi SL and Rennick KA
Journal of Chromatography A, 913(1-2), 379-386 (2001)
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Questions

1–3 of 3 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Should it be stored at 4℃??

    1 answer

    1. This product may be stored at room temperature. For products with no specified storage temperature given on the label or product page, ambient storage may be assumed. Products that require controlled temperature storage, such as refrigeration or freezer conditions, storage temperatures are published on the product label, Certificate of Analysis, the Safety Data Sheet (SDS).

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