Data is not available to indicate whether DNA contamination could affect sequencing. However, including DNase treatment is recommended to minimize genomic DNA in the sequencing reads.
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
1 kit | Disponible para enviar HOYdesdeMILWAUKEE | $1,560.00 |
Acerca de este artículo
usage
sufficient for 250 purifications
Quality Segment
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product, Sustainability features
technique(s)
RNA purification: suitable
test parameters
: ~30 min kit run time, sample volume: 100 mg
storage temp.
15-25°C
General description
Después de moler el tejido hasta un polvo fino en nitrógeno líquido, se lisan las células, y los restos celulares se separan física y químicamente del ARN endógeno. A continuación el ARN se une a un sustrato de sílice sustentado en columna y varias etapas de lavado eliminan los contaminantes remanentes.
El ARN total es eluido de la columna y utilizado en las aplicaciones habituales como inmunotransferencias Northern, RT-PCR y qRT-PCR.
Application
- para extraer ARN de material molido en nitrógeno líquido
- para aislar el ARN total de las plántulas mutantes y wt (naturales)
- para extraer el ARN total de muestras de hojas
- para extraer ARN de todos los tejidos vegetales
Features and Benefits
- Produce hasta 60 μg de ARN concentrado puro por preparación
- Protocolo eficiente que permite la purificación del ARN en 30 minutos o menos
- Especialmente diseñado para la investigación con tejidos vegetales difíciles
Legal Information
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Este artículo | |||
|---|---|---|---|
| description sufficient for 250 purifications | description sufficient for 50 purifications | description sufficient for 10 purifications | description sufficient for 70 purifications |
| technique(s) RNA purification: suitable | technique(s) RNA purification: suitable | technique(s) RNA purification: suitable | technique(s) DNA purification: suitable |
| usage sufficient for 250 purifications | usage sufficient for 50 purifications | usage sufficient for 10 purifications | usage sufficient for 70 purifications |
| greener alternative category | greener alternative category | greener alternative category , Aligned | greener alternative category , Aligned |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
| greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Inherently Safer Chemistry for Accident Prevention |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
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Solo componentes del kit
- Binding Solution
- Lysis Solution
- Wash Solution 1
Los componentes del kit también están disponibles por separado
- 2-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) 2
Palabra de advertencia
Danger
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
Órganos diana
Liver,Heart
Clase de almacenamiento
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
¿Qué está pasando?
WGK 3
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I’m using your plant RNA extraction kit for RNA-seq. If I skip the DNase treatment, could DNA contamination affect the sequencing library, or is it mandatory to include this step? Thank you.
1 answer-
Helpful?
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does it sufficient for small RNA and/or non-coding RNA extraction?
1 answer-
Yes, this kit is sufficient for small RNA. However, It is worth noting that small RNA molecules, such as microRNA, siRNA, tRNA, 5S rRNA, are not efficiently recovered using the standard procedure. If desired, these small RNA molecules can be recovered by increasing the amount of binding solution, as described in Protocol A under Procedure Step 4.
For more information, please see the protocol associated with this product:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/323/177/strn250bul.pdfHelpful?
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