SRP3003

Activin A human

recombinant, expressed in E. coli, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture

• Sinónimos: Activin beta-A, FRP, Inhibin beta-1
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• origen biológico: human
• recombinante: expressed in E. coli
• Ensayo: ≥97% (HPLC); ≥97% (SDS-PAGE)
• Formulario: lyophilized
• potencia: ≤2.0 ng/mL ED₅₀
• mol peso: 26.0 kDa
• envase: pkg of 10 μg
• técnicas: cell culture | mammalian: suitable
• impurezas: <0.1 EU/μg endotoxin, tested
• Nº de acceso UniProt: P08476
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... INHBA(3624) ; mouse ... Inhba(16323) ; rat ... Inhba(29200)

Descripción

Descripción general

Activins (also referred to as inhibins) are generally dimers of three subunits: inhibin βA, inhibin βB, and inhibin α.[1] Recombinant human Activin A (inhibin βA) is a 26kDa disulfide-linked homodimer of two bA chains, each containing 116 amino acid residues. The sequence of mature Activin A is completely homologous between human, mouse, and rat species.

Aplicación

Activin A has been used in monolayer induction for endoderm (hepatocyte) differentiation.[2]

Activin A human has been used to study rRNA gene transcription during directed differentiation of human embryonic stem cells.[3]

Acciones bioquímicas o fisiológicas

Activin A (inhibin βA) is a TGF (transforming growth factor)-β family member that exhibits a wide range of biological activities including regulation of cellular proliferation and differentiation, and promotion of neuronal survival.[4] Elevated levels of Activin A in human colorectal tumors and in postmenopausal women have been implicated in colorectal and breast cancers, respectively.[1][5] Activin A plays a central role in reproduction by regulating the levels of follicle stimulating hormone (FSH). It is involved in hematopoiesis, embryogenesis and osteogenesis. It exhibits both tumor suppressing and promoting effects.[6] In underweight and overweight humans, high levels of Activin A in serum might be associated with the pathogenesis of asthma.[7] The biological activities of Activin A can be neutralized by inhibins and by the diffusible TGF-β antagonist, follistatin.[8]

Secuencia

GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS

Forma física

Lyophilized with no additives.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable