SRP2119

RNA Polymerase II, RPB12 subunit, GST tagged human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• Sinónimos: ABC10-alpha, RPABC4, RPB10alpha, RPB12, RPB7.0, hRPB7.0, hsRPB10a
• Código UNSPSC: 12352202
• NACRES: NA.26

Propiedades

• origen biológico: human
• recombinante: expressed in E. coli
• Ensayo: ≥80% (SDS-PAGE)
• Formulario: frozen liquid
• mol peso: ~33 kDa
• envase: pkg of 10 μg
• condiciones de almacenamiento: avoid repeated freeze/thaw cycles
• concentración: 100 μg/mL
• color: clear colorless
• Nº de acceso NCBI: NM_005034
• Nº de acceso UniProt: P53803
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... POLR2K(5440)

Descripción

Descripción general

POLR2K (RNA polymerase II subunit K) is located on human chromosome 8q22. Rpb12 is the eukaryotic subunit that is also called as ABC10α. Rpb12 is the general subunit of all three eukaryotic RNA polymerases.

Acciones bioquímicas o fisiológicas

RPB12 (also called RPB10 ) is shared between all three RNA polymerases and is homologous to the archaeal P subunit of RNA polymerase. Like RPB10, it is essential for yeast cell viability. RPB12 is also a zinc-binding protein with a canonical tetra-coordinating zinc motif but binds zinc in vitro much less efficiently than the RPB10 subunit. It has been shown to interact with both RPB1 and RPB2 in vitro and the human subunit can functionally substitute for its yeast counterpart.

RPB12/RPC10 (RNA polymerase II subunit K) is required for growth in Saccharomyces cerevisiae. The carboxy-terminal domain (CTD) of RNAP II plays a major role in recruiting many of these activities to the transcriptional machinery.

Forma física

Clear and colorless frozen liquid solution

Nota de preparación

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable