SML3351

Br-DAPI hydrochloride

≥95% (HPLC)

• Sinónimos: 2-[4-(Aminoiminomethyl)phenyl]-3-bromo-1H-indole-6-carboximidamide hydrochloride, 3-Bromo-2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide
• Fórmula empírica (notación de Hill): C₁₆H₁₄BrN₅ · xHCl
• Peso molecular: 356.22 (free base basis)
• UNSPSC Code: 41116158
• NACRES: NA.77

Propiedades

• Quality Level: 100
• assay: ≥95% (HPLC)
• form: powder
• storage condition: desiccated
• color: white to beige
• solubility: DMSO: 2 mg/mL, clear
• storage temp.: 2-8°C
• SMILES string: BrC1=C(NC2=CC(C(N)=N)=CC=C12)C3=CC=C(C=C3)C(N)=N

Descripción

Biochem/physiol Actions

Br-DAPI is a DAPI-derived DNA-binding photosensitizer that produces reactive oxygen species (ROS) upon irradiation, resulting in single/double stranded breaks of bound DNA. Light-induced viability reduction is observed among A549 cells treated with Br-DAPI (IC50 = 1.3 μM; 45 min treatment), but not DAPI (IC50 >64 μM) upon photosensitization (365 nm 15 mW/cm2, 4.5 J/cm2 fluence rate). Its high permeation in both Gram-negative and Gram-positive bacteria allows selective light-induced eradication of bacteria over mammalian cells at low concentration (IC50 = 0.29 μM/N99 E. coli and 0.5 μM/B. subtilis) and light intensity (5 min irradiation by 365 nm LED at 15 mW/cm2, 4.5 J/cm2).

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable