SAB4200641

Monoclonal Anti-Brg1 antibody produced in rat

clone 3G4, purified from hybridoma cell culture

• Sinónimos: Anti-ATP-dependent helicase SMARCA4, Anti-BAF190, Anti-BAF190A, Anti-BRG1, Anti-BRG1-associated factor 190A, Anti-BRM/SWI2-related gene 1, Anti-MRD16, Anti-RTPS2, Anti-SMARCA4, Anti-SNF2, Anti-SNF2-beta, Anti-SNF2-like 4, Anti-SNF2L4, Anti-SNF2LB, Anti-SWI/SNF related, Anti-SWI2, Anti-actin dependent regulator of chromatin, Anti-brahma protein-like 1, Anti-global transcription activator homologous sequence, Anti-hSNF2b, Anti-homeotic gene regulator, Anti-matrix associated, Anti-member 4, Anti-mitotic growth and transcription activator, Anti-nuclear protein GRB1, Anti-protein BRG-1, Anti-protein brahma homolog 1, Anti-subfamily a, Anti-sucrose nonfermenting-like 4, Anti-transcription activator BRG1
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 3G4, monoclonal
• Formulario: buffered aqueous solution
• mol peso: ~185 kDa
• reactividad de especies: mouse, human
• concentración: ~1 mg/mL
• técnicas: flow cytometry: 2.5-5 μg/test using HeLa cells; immunoblotting: 0.1-0.2.0 μg/mL using HeLa nuclear extract; immunofluorescence: 10 μg/mL using HeLa cells
• isotipo: IgG1
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... BRG1(6597) ; mouse ... Brg1(20586)

Descripción

Descripción general

Monoclonal Anti-Brg1 (rat IgG1 isotype) is derived from the hybridoma 3G4 produced by the fusion of mouse myeloma cells (SP2) and lymph node cells from rat immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of human Brm-related gene-1 (Brg1) protein. Brg1 or SWI/SNF (SWItch/Sucrose non-fermentable) related, matrix-associated, actin-dependent, regulator of chromatin, subfamily A, member 4 (SMARCA4) gene with 35 exons, is encoded by the gene mapped to human chromosome 19p13.

Inmunógeno

a synthetic peptide corresponding to a sequence at the C-terminal region of human Brg1 protein

Aplicación

Monoclonal Anti-Brg1 antibody produced in rat has been used in:

• immunoblotting (~185 kDa)
• flow cytometry
• immunofluorescence

Acciones bioquímicas o fisiológicas

Brm-related gene-1 (Brg1) (or hBrm) protein is the central catalytic ATPase of the SWI/SNF chromatin-remodeling complex, which is involved in transcriptional modulation of hormone-responsive promoters through binding of the complex to various nuclear receptors and its recruitment to gene-specific promoters. In addition, BRG1 has also been shown to act as a transcriptional repressor. It interacts with retinoblastoma tumor suppressor to form a repressor complex which inhibits cell cycle proteins such as cyclins A, D1 and E. BRG1 participates in the firing of origins and the process of replication elongation. This function of BRG1 also facilitates embryogenesis. In addition, It functions as a tumor suppressor and maintains genome stability and prevent cancer.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable