The reconstituted protease inhibitor solution may be stored at 2-8 °C for at least 2 weeks. Long term solution stability studies at -20°C have not been performed, however 10X solutions - 10mL per tablet - will be stable for at least 1 month in frozen aliquots. Avoid repeated freeze/thaw cycles.
S8830
Protease Inhibitor Cocktail
SIGMA FAST, EDTA-Free, tablet, for the inhibition of serine, cysteine, aspartic and metalloproteases, for use in purification of Histidine-tagged proteins
Sinónimos:
Cóctel de inhibidores de proteasas
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About This Item
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Nombre del producto
Comprimidos de cóctel de inhibidores de proteasas SIGMAFAST™ sin EDTA, for use in purification of Histidine-tagged proteins
biological source
bovine lung
Quality Level
form
tablet
storage temp.
2-8°C
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General description
Specificity
Application
Features and Benefits
Pepstatina A: contienen una formulación patentada exclusiva, con una sal de pepstatina A para proporcionar una protección añadida que normalmente no se obtiene en un formato de comprimido.
Especificidad amplia: inhibe las proteasas de serina, cisteína y aspártico, y las metaloproteasas, proporcionando una amplia protección a las muestras proteicas.
Alto rendimiento: un comprimido produce 100 ml de la combinación, y se recomienda para la inhibición de las proteasas presentes en un máximo de 20 g de masa celular en 100 ml de extracto.
Estabilidad prolongada: los comprimidos son estables tal como se suministran durante un mínimo de 2 años a 2–8 °C, y la disolución de inhibidores de proteasa reconstituida (1´ o 10´) es estable por lo menos durante 2 semanas a 2–8 °C.
Components
Bestatina
E-64
Pepstatina A
Fosforamidon
Leupeptina
Aprotinina
Quantity
Physical form
Storage and Stability
Legal Information
also commonly purchased with this product
related product
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1A
Storage Class
8A - Combustible corrosive hazardous materials
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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Artículos
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
Contenido relacionado
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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Can the reconstituted protease inhibitor cocktail solution be stored at -20°C and for how long?
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What is the difference between Protease Inhibitors P8849 and S8830?
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P8849 is a DMSO solution and S8830 is a water soluble tablet. P8849 formulation is considered proprietary but does contain AEBSF, Pepstatin A, Bestatin, E-64, and Phosphoramidon. The formulation of S8830 also contains Aprotinin and Pepstatin A and the exact formulation is given on the product information sheet (under Documents, above).
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How should I dissolve Product No. S8830, SIGMAFAST™ Protease Inhibitor?
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You can dissolve the product in 100 ml of water to make a 1X solution. You can make a 10X stock solution using 10 ml of water and then dilute 1:10 for use. Concentrations greater than 1X may appear slightly hazy. This will not affect the performance of the protease inhibitors.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Product No. P8849, Protease Inhibitor Cocktail, be used with with HIS-Select™ products or any other immobilized metal affinity chromatography (IMAC) products?
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This product does not contain EDTA and can be used with sample processing by immobilized metal affinity chromatography (IMAC) suchas used for the purification of histidine-tagged proteins..
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Are there publications that cite use of Product S8830, SIGMAFAST™ Protease Inhibitor Cocktail Tablets?
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Yes. Several examples of published papers that cite the use of Product S8830 include these:1. Smith, E.R., et al., Instability of fibroblast growth factor-23 (FGF-23): Implications for clinical studies. Clinica Chimica Acta, 412(11-12), 1008-1011 (2011). Use was with blood at 20 μL cocktail per 1 mL of blood.2. Reber, S., "Isolation of Centrosomes from Cultured Cells" (Chapter 8), from Microtubule Dynamics: Methods in Molecular Biology (Springer Protocols), vol. 777, pp. 107-116 (2011).
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