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S8761

Sigma-Aldrich

Sodium Bicarbonate

Solution (7.5%), sterile-filtered, suitable for cell culture

Sinónimos:

aqueous sodium bicarbonate

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About This Item

Número de CAS:
144-55-8
MDL number:
MFCD00003528
UNSPSC Code:
12161702
PubChem Substance ID:
24899815
NACRES:
NA.75

product name

Sodium bicarbonate solution, solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture

sterility

sterile-filtered

product line

BioReagent

form

solution (7.5%)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

7.8-8.2

storage temp.

2-8°C

SMILES string

[Na+].OC([O-])=O

InChI

1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1

InChI key

UIIMBOGNXHQVGW-UHFFFAOYSA-M

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General description

Sodium bicarbonate (NaHCo3) is a commonly used alkaline buffering agent(5) in laboratories. The addition of this buffer to the cell culture medium aids in maintaining the appropriate pH by using the carbon dioxide present in the incubator. In addition to its buffering properties, Sodium bicarbonate also acts as a source of sodium and aids in sodium gluconate synthesis during the fermentation process.

Application

Sodium bicarbonate solution has been used:
  • as a component of Hanks′ buffered salt solution (HBSS) and recording medium for culturing of tissue explants from rats or mice
  • as a component of Dulbecco′s modified Eagle′s medium (DMEM) to culture mice tissue explants
  • as a component of Minimum Essential Media (MEM) to study the cytotoxicity against human colorectal adenocarcinoma cells and mammary gland adenocarcinoma
  • to prepare collagen mix droplets for 3D collagen sprouting assay
  • to prepare collagen matrix for rat epidermal keratinocyte (REK) organotypic culture

Preparation Note

Prepared in cell culture grade water.

related product

Referencia del producto
Descripción
Precios

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Phenotypic Modulation of Adipose-Derived Stem Cells and Fibroblasts Treated with Povidone--Iodine and Chlorhexidine in Mono and Coculture Models
Chelmu? BA, et al.
Biomedicines, 11(7), 1855-1855 (2023)
Nicoline Dorothea Jakobsen et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 178, 106282-106282 (2022-08-23)
Rat epidermal keratinocyte (REK) Organotypic culture (ROC) is an epidermis model that is robust and inexpensive to develop and maintain, and it has in previous studies been shown to have permeability characteristics close to those of human skin. Here, we
Mette Flinck et al.
Cell cycle (Georgetown, Tex.), 17(9), 1056-1067 (2018-06-14)
Precise acid-base homeostasis is essential for maintaining normal cell proliferation and growth. Conversely, dysregulated acid-base homeostasis, with increased acid extrusion and marked extracellular acidification, is an enabling feature of solid tumors, yet the mechanisms through which intra- and extracellular pH
Anna E Engler et al.
Cell reports, 33(13), 108553-108553 (2020-12-31)
There is an increasing appreciation for the heterogeneity of myeloid lineages in the lung, but relatively little is known about populations specifically associated with the conducting airways. We use single-cell RNA sequencing, flow cytometry, and immunofluorescence to characterize myeloid cells
Wenqing Zhou et al.
Journal of cell science, 133(17) (2020-08-14)
Neutrophils rely on glycolysis for energy production. How mitochondria regulate neutrophil function is not fully understood. Here, we report that mitochondrial outer membrane protein Mitofusin 2 (MFN2) regulates neutrophil homeostasis and chemotaxis in vivo Mfn2-deficient neutrophils are released from the
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Protocolos

Cultrex® 3-D Culture Matrix™ Rat Collagen I Protocol

3-D Culture Matrix™ Collagen I facilitates cellular growth and differentiation in vitro, offering versatile applications in biomedical research.

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