S7199

Monoclonal Anti-SERCA3 antibody produced in mouse

~1.0 mg/mL, clone PL/IM430, purified immunoglobulin, buffered aqueous solution

• Sinónimos: Anti-ATP2A3, Anti-ATPase, Ca(2+)-Transporting, Ubiquitous, Anti-Sarcoplasmic reticulum Ca(2+)-ATPase 3
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• conjugado: unconjugated
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: PL/IM430, monoclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~100 kDa
• reactividad de especies: human
• concentración: ~1.0 mg/mL
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.25-0.5 μg/mL using platelet total cell extract.
• isotipo: IgG1
• Nº de acceso UniProt: Q93084
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ATP2A3(489)

Descripción

Descripción general

Monoclonal Anti-SERCA3 (mouse IgG1 isotype) is derived from the hybridoma PL/IM430 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. The ATPase sarcoplasmatic/endoplasmatic reticulum Ca²⁺ transporting 3 (ATP2A3) gene codes for Ca²⁺-ATPase3 from the sarco/endoplasmic reticulum (SERCA3) and the coded protein is expressed in endothelial and epithelial tissues. The human SERCA3 gene gives rise to six 3¢-end splice variants encoding proteins varying in their C-terminal part and termed SERCA3a-f. SERCA3 gene is mapped to human chromosome 17p13.3.

Especificidad

Monoclonal Anti-SERCA3 recognizes human SERCA3.

Aplicación

Monoclonal Anti-SERCA3 antibody produced in mouse may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunocytochemistry
• western blotting
• immunoprecipitation
• immunohistochemistry

Acciones bioquímicas o fisiológicas

Sarco/endoplasmic calcium ATPase pumps (SERCA) protein plays a pivotal role in intracellular calcium [Ca²⁺] homeostasis by lowering the cytoplasmic [Ca²⁺] concentration. Expression of sarco/endoplasmic reticulum (SERCA3) gene is reduced in gastric/colon carcinoma and leukemia cell types.The ATPase sarcoplasmatic/endoplasmatic reticulum Ca²⁺ transporting 3 (ATP2A3) gene may serve as an important target for salinomycin. Overexpression of this gene stimulates apoptosis. SERCA3 protein plays an important role in Ca²⁺ transport.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)