S6446

Anti-SMC1L1 (C-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Sinónimos: Anti-SMC1, Anti-SMC1 structural maintenance of chromosomes 1-like 1, Anti-SMC1α
• Número MDL: MFCD03791776
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• conjugado: unconjugated
• forma del anticuerpo: IgG fraction of antiserum
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• mol peso: antigen 160 kDa
• reactividad de especies: rat (predicted), human, mouse (predicted)
• técnicas: immunoprecipitation (IP): 5-10 μL using HEK 293-T cell lysates; indirect immunofluorescence: 1:100-1:200 using paraformaldehyde-fixed HEK 293-T cells; microarray: suitable; western blot: 1:500-1:1,000 using HeLa nuclear extracts
• Nº de acceso UniProt: Q14683
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... SMC1A(8243) ; mouse ... Smc1a(24061) ; rat ... Smc1a(63996)

Descripción

Descripción general

Structural maintenance of chromosomes protein 1A (SMC1A, SMC1L1, SMC1), a structural maintenance of chromosomes (SMC) protein belongs to the SMC superfamily and is an important constituent of the cohesin complex. This gene is located on human chromosome Xp11.22.

Especificidad

Anti SMC1L1 (also known as SMC1 and SMC1a) specifically recognizes SMC1L1.

Inmunógeno

synthetic peptide corresponding to amino acids 1210-1225 of human SMC1L1, conjugated to KLH via an N-terminal cysteine residue. The immunizing sequence is conserved in mouse and rat.

Aplicación

Anti-SMC1L1 (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Acciones bioquímicas o fisiológicas

Structural maintenance of chromosomes protein 1A (SMC1A, SMC1L1, SMC1) can be phosphorylated by Ataxia-telangiectasia, mutated (ATM) indicating a potential role for this protein in DNA repair. Thus, SMC1L1 phosphorylation appears to be the critical downstream event in the ATM-nibrin (NBS1)-breast cancer type 1 (BRCA1) pathway, which mediates cell survival and chromosomal stabilization after DNA damage. Mutations in SMC1L1 gene result in the Cornelia de Lange syndrome, a multisystem developmental disorder. It participates in chromosome dynamics, double-strand break (DSB) repair and tumorigenesis. This gene can control the cell cycle and helps to maintain the stability of genome. Suppression of SMC1A can block the proliferation and migration of hepatocellular carcinoma cells.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)