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S0937

Sucrose Phosphorylase

recombinant, expressed in E. coli, lyophilized powder, ≥45 units/mg solid

Sinónimos:

SPase, disaccharide glucosyltransferase, sucrose glucosyltransferase, Sucrose:orthophosphate α-D-glucosytransferase

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Talla/SKUDisponibilidadPrecio
250 units
Comprobar disponibilidad del carrito
$250.00
1000 units
Comprobar disponibilidad del carrito
$827.00
$702.95

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Número CAS:
9074-06-0
UNSPSC Code:
12352204
NACRES:
NA.54
Número CE:
2.4.1.7 (BRENDA, IUBMB)
MDL number:
MFCD00132397
Specific activity:
≥45 units/mg solid
Recombinant:
expressed in E. coli

$250.00

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recombinant

expressed in E. coli

Quality Segment

form

lyophilized powder

specific activity

≥45 units/mg solid

mol wt

56 kDa by SDS-PAGE

shipped in

wet ice

storage temp.

−20°C

General description

Research area: Cell signaling
Sucrose Phosphorylase belongs to glycoside hydrolase, GH13 family. It comprises of four domains with the glucose anomeric carbon-binding site and a glucoside-binding site. The active site residues include Asp192 and Glu232. It is majorly produced by bifidobacteria and lactic acid bacteria. The cross-linked sucrose phosphorylase aggregates is thermostable and could be exploited for industrial catalysis of glycosylation.

Application

Sucrose Phosphorylase has been used in sucrose determination in wheat plant and in sucrose hydrogen production.
Sucrose phosphorylase has been used:

  • To assess the enzymatic synthesis of stable, odorless, and powdered furanone glucosides.
  • To investigate the novel transglucosylating reaction with carboxylic compounds.
  • In sucrose determination in wheat plant and in sucrose hydrogen production.

Biochem/physiol Actions

Sucrose phosphorylase catalyzes the reversible conversion of sucrose (α-D-glucopyranosyl-1,2-β-D-fructofuranoside) and phosphate into D-fructose and α-D-glucose 1-phosphate. This reaction plays a crucial role in generating the vital glucose component through sucrose metabolism.(1)

Physical form

Contains sucrose as stabilizer.

Other Notes

One unit will produce 1.0 μmole of D-fructose from sucrose per min with the corresponding reduction of NADP to NADPH at pH 7.6, at 25 °C.

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Este artículo
16104718033721891
Sucrose Phosphorylase recombinant, expressed in E. coli, lyophilized powder, ≥45 units/mg solid

Sigma-Aldrich

S0937

Sucrose Phosphorylase

Sacarosa puriss., meets analytical specification of Ph. Eur., BP, NF

Sigma-Aldrich

16104

Sacarosa

Sucrose solution suitable for NMR (reference standard), 2 mM in H2O:D2O (9:1) (99.9 atom % D), sodium azide (trace), sodium chloride 0.25 M

Sigma-Aldrich

718033

Sucrose solution

Sucrose solution suitable for NMR (reference standard), 2 mM in H2O:D2O (9:1) (99.9 atom % D), DSS 0.5 mM, NMR tube size 3 mm × 8 in.

Supelco

721891

Sucrose solution

description

recombinant, expressed in E. coli, lyophilized powder, ≥45 units/mg solid

description

puriss., meets analytical specification of Ph. Eur., BP, NF

description

suitable for NMR (reference standard), 2 mM in H2O:D2O (9:1) (99.9 atom % D), sodium azide (trace), sodium chloride 0.25 M

description

suitable for NMR (reference standard), 2 mM in H2O:D2O (9:1) (99.9 atom % D), DSS 0.5 mM, NMR tube size 3 mm × 8 in.

specific activity

≥45 units/mg solid

specific activity

-

specific activity

-

specific activity

-

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

form

lyophilized powder

form

solid

form

solution

form

-

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

-

storage temp.

-

storage temp.

-

mol wt

56 kDa by SDS-PAGE

mol wt

-

mol wt

-

mol wt

-

Pictogramas

Health hazard
GHS08

Palabra de advertencia

Danger

Códigos de peligro

H334

Indicaciones de precaución

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Clase de almacenamiento

11 - Combustible Solids

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