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RAB0480

Rat Tumor Necrosis Factor α ELISA Kit

for cell and tissue lysates

Sinónimos:

Cachectin, TNF-alpha, Tumor necrosis factor alpha

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A ustedes/SKUDisponibilidadPrecio
1 kit
Normalmente se envía en 1 semana. (Para pedidos fuera de Estados Unidos y Europa, calcule la entrega 1 o 2 semanas más tarde)
$608.00

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NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
rat ... Tnf(24835)
Input:
sample type cell lysate
sample type tissue lysate
Species reactivity:
rat
Storage temp.:
−20°C
Shipped in:
wet ice

$608.00


Normalmente se envía en 1 semana. (Para pedidos fuera de Estados Unidos y Europa, calcule la entrega 1 o 2 semanas más tarde)

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species reactivity

rat

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type cell lysate
sample type tissue lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 82.3-20000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... Tnf(24835)

General description

The Rat TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TNF-α cell lysate and tissue lysate.

Immunogen

Recombinant Rat TNF-α

Application

For research use only. Not for use in diagnostic procedures.
Rat Tumor Necrosis Factor α ELISA Kit has been used to measure rat tumor necrosis-α (TNF-α) in cell lysate and tissue lysate.

Biochem/physiol Actions

Tumor necrosis factor-α (TNF-α) affects various cellular processes such as blood-brain barrier, inflammatory, thrombogenic, and vascular changes following brain damage. TNF-α expression is upregulated in various central nervous system disorders, including Alzheimer′s disease, multiple sclerosis, Parkinson′s disease, meningococcal meningitis and HIV infection. Elevated expression of TNF-α in brain and blood in response to lipopolysaccharide might increase brain stem thrombosis and hemorrhage and stroke sensitivity/risk in hypertensive rats. Tumor necrosis factor-α activates polymorphonuclear leukocyte NADPH oxidase, thereby facilitates development of systemic oxidative stres (OS), inflammation and hypertension in rats.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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Este artículo
RAB0479RAB0340RAB0478
description

for cell and tissue lysates

description

for serum, plasma and cell culture supernatant

description

for serum, plasma, cell culture supernatants and urine

description

for serum, plasma and cell culture supernatant

species reactivity

rat

species reactivity

rat

species reactivity

human

species reactivity

porcine

Gene Information

rat ... Tnf(24835)

Gene Information

rat ... Tnf(24835)

Gene Information

human ... LTA(4049)

Gene Information

Porcine ... TNF(397086)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 82.3-20000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 82.3-20000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 60 pg/mL
standard curve range: 74.07-18000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 20 pg/mL
standard curve range: 0.014-10 ng/mL

input

sample type cell lysate
sample type tissue lysate

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

input

sample type cell culture supernatant(s)
sample type plasma
sample type urine
sample type serum

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric


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Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS & Precios

  • ELISA 5X Sample Diluent Buffer (Item D2)
    SDS

  • ELISA 5X Assay/Sample Diluent Buffer E (Item E2)
    SDS

  • 2X Cell Lysis Buffer (Item J)
    SDS

  • ELISA Stop Solution (Item I)
    SDS

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS

  • 20X Wash Buffer (Item B)
    SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Clase de almacenamiento

10 - Combustible liquids



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Certificados de análisis (COA)

Lot/Batch Number

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Questions

  1. how many tests can be done by a kit

    1 answer
    1. The Rat TNF-alpha ELISA kit provides one 96-well plate. A set of 7 standards is loaded in duplicates to generate a standard curve, leaving 82 wells for testing 41 samples in duplicates.

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