This product detects both pro and mature human brain-derived neurotrophic factor (BDNF), but cannot differentiate. In reference to free or bound forms of the mature BDNF, epitope mapping has not been performed and the specific binding site has not been identified. The immunogen is aa128-247 (Accession# P23560).
RAB0026
Human BDNF ELISA Kit
for serum, plasma, cell culture supernatant and urine
Sinónimos:
BDNF, Brain-derived neurotrophic factor
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
1 kit | Normalmente se envía en 1 semana. (Para pedidos fuera de Estados Unidos y Europa, calcule la entrega 1 o 2 semanas más tarde) | $567.00 |
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NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... BDNF(627)
Input:
sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type urine
sample type serum
sample type cell culture supernatant(s)
sample type urine
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice
$567.00
Normalmente se envía en 1 semana. (Para pedidos fuera de Estados Unidos y Europa, calcule la entrega 1 o 2 semanas más tarde)
Servicio técnico
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Permítanos ayudarlespecies reactivity
human
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable, capture ELISA: suitable
input
sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type urine
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 80 pg/mL
standard curve range: 0.066-16 ng/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... BDNF(627)
General description
The brain derived neurotrophic factor (BDNF) gene is mapped to human chromosome 11p14.1. BDNF is a member of the neurotrophin family of growth factors. The gene encodes a precursor protein, proBDNF. Mature BDNF (mBDNF) is synthesized by post-translational cleavage of proBDNF. Both proBDNF and mBDNF play crucial roles in cellular signaling.
The Human BDNF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BDNF in serum, plasma, cell culture supernatants and urine.
Immunogen
Recombinant Human BDNF
Application
For research use only. Not for use in diagnostic procedures.
Human BDNF ELISA kit has been used to determine the effect of bone marrow mesenchymal stem cell (MSC) transplantation on BDNF protein expression in the striatum of Tourette syndrome (TS) rats. It has also been used to determine the plasma BDNF levels in GD1 (Gaucher′s disease type 1) patients and control population.
Human BDNF ELISA kit has been used to determine the effect of bone marrow mesenchymal stem cell (MSC) transplantation on BDNF protein expression in the striatum of Tourette syndrome (TS) rats. It has also been used to determine the plasma BDNF levels in GD1 (Gaucher′s disease type 1) patients and control population.
Human BDNF ELISA Kit has been used:
- to measure the brain-derived neurotrophic factor (BDNF) protein expression
- to determine BDNF level in the slice culture medium and the extracts of organotypic brain slice cultures (OBSCs)
- to determine BDNF level in plasma
Biochem/physiol Actions
Brain derived neurotrophic factor (BDNF) is involved in hippocampal function and verbal episodic memory in humans. Hence, variation in the BDNF gene expression alters these neurological functions. BDNF also plays a vital role in vascular function and participates in angiogenesis via the specific receptor tropomyosin-related kinase B (TrkB). It is involved in the pathogenesis of Alzheimer′s disease. ProBDNF interacts with p75 neurotrophin receptor, leading to long-term depression in the hippocampus.
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
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Este artículo | |||
|---|---|---|---|
| description for serum, plasma, cell culture supernatant and urine | description for cell culture supernatants, plasma, and serum samples | description for serum, plasma and cell culture supernatants | description affinity isolated antibody, lyophilized powder |
| species reactivity human | species reactivity human, rat | species reactivity rhesus macaque | species reactivity mouse, human, rat |
| Gene Information human ... BDNF(627) | Gene Information rat ... Bdnf(24225) | Gene Information - | Gene Information human ... BDNF(627) |
| assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range - |
| input sample type plasma | input sample type plasma | input - | input - |
| detection method colorimetric | detection method colorimetric | detection method - | detection method - |
| technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) western blot: 1:400 using recombinant mouse proBDNF, western blot: 1:200 using rat glioma C6 and human neuroblastoma SH-SY5Y cell lines |
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS & Precios
Palabra de advertencia
Warning
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Met. Corr. 1
Clase de almacenamiento
10 - Combustible liquids
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Which isoform is identified?
1 answer-
Helpful?
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Could you please confirm if this kit is capable of recognizing both free and bound BDNF?
1 answer-
Epitope mapping has not been conducted, so the exact binding site is unknown. However, the immunogen is located at aa128-247 (Accession# P23560).
Helpful?
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