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MilliporeSigma

R7020

Sigma-Aldrich

Transcript RNA Markers 0.2-10 kb

for RNA electrophoresis

Sinónimos:

RNA agarose gel electrophoresis ladder, RNA agarose gel electrophoresis marker, northern blot ladder, northern blot marker, ssRNA ladder, ssRNA marker, tRNA ladder, tRNA marker

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About This Item

UNSPSC Code:
41105337
NACRES:
NA.25

grade

for molecular biology

Quality Level

form

liquid

usage

25 uses

suitability

suitable for electrophoresis (RNA)

storage temp.

−70°C

General description

Sigma′s Transcript RNA Marker (0.2 – 10 kb) contains 9 RNA transcripts from 200 – 10,000 bases. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.

Application

Suitable for size determination of small RNA using native or denaturing agarose gel electrophoresis.
Transcript RNA Markers 0.2-10 kb has been used as a molecular weight marker for northern blot hybridization.

Components

Sigma′s Transcript RNA Marker (0.2 – 10 kb) is provided in a gel-loading solution with 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains nine defined RNA transcripts, 200–10,000 nucleotides
For optimal resolution, the recommended agarose gel concentration is 1.0%.

related product

Referencia del producto
Descripción
Precios

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Protocolos

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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