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R5258

Sigma-Aldrich

Riboflavin Binding Protein from chicken egg white

lyophilized powder

Sinónimos:

Binding Protein, Chicken Egg White Protein, Riboflavin Protein

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2 MG
$64.98
10 MG
$187.00

$64.98

Precio de catálogo$72.20Ahorre 10 %

Fecha estimada de envío22 de abril de 2025

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2 MG
$64.98
10 MG
$187.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.26

$64.98

Precio de catálogo$72.20Ahorre 10 %

Fecha estimada de envío22 de abril de 2025

biological source

chicken egg white

Quality Level

200

form

lyophilized powder

concentration

≥50% protein (biuret)

UniProt accession no.

P02752

storage temp.

−20°C

Gene Information

chicken ... RBP(396449)

General description

Riboflavin Binding Protein (RBP) comprises of 19 cysteines and corresponds to a molecular weight of 30 kDa.[1] Egg white RBP is produced in the oviduct cells. Chicken egg white RBP contains an N-terminal ligand binding domain and a C-terminal phosphorylated motif.[2]
Riboflavin binding protein (RBP) transports riboflavin through the bloodstream of the laying hen into the eggs, where the vitamin is essential for embryonic growth and development. RBP consists of a single polypeptide chain containing 9 disulfide bonds. It is a glycoprotein containing ~14% carbohydrate, with sugar moieties including galactose, mannose and glucosamine, and is phosphorylated.

Application

Riboflavin Binding Protein from chicken egg white may be used in fluorescence quenching experiments.[1]

Biochem/physiol Actions

Riboflavin Binding Protein (RBP) is crucial for normal embryo development.[1] RBP interacts with riboflavin and its analogs. The RBP-riboflavin complex is stabilized by interaction between the isoalloxazyne ring of riboflavin and the tyrosine 75 and tryptophan 156 of residues of RBP.[3] RBP elicits sweet supressing functionality and may interact with the sweet taste receptor.[4]
Riboflavin binding protein binds to flavokinase and regulates its activity. [5]

Preparation Note

Supplied as the riboflavin-depleted apo-form.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
014H7040
113H7140
083H7035

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Riboflavin-binding protein exhibits selective sweet suppression toward protein sweeteners
Maehashi K, et al.
Chemical Senses, 32(2), 183-190 (2006)
The effect of riboflavin binding protein (RPB) on flavokinase catalytic activity.
Slomczynska, M.
Comparative Biochemistry and Physiology, 87, 681-685 (1987)
Electrochemistry of riboflavin-binding protein and its interaction with riboflavin
Bartovsik M, et al.
Bioelectrochemistry, 76(1-2), 70-75 (2009)
Crystal structure of chicken riboflavin-binding protein
Monaco HL
The Embo Journal, 16(7), 1475-1483 (1997)
Binding study of riboflavin-binding protein with riboflavin and its analogues by differential scanning calorimetry
Wasylewski M
Journal of Protein Chemistry, 19(6), 523-528 (2000)
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