PP2386
Yeast Tag Vector Set
plasmid vectors for molecular cloning
Sinónimos:
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
About This Item
Productos recomendados
conjugado
E-Tag
S-Tag
streptavidin conjugate
etiqueta
10-His tagged
FLAG® tagged
GST tagged
Glu-Glu tagged
Hemagglutinin A (HA) tagged
Herpes Simplex Virus (HSV) tagged
T7 tagged
V5 tagged
c-Myc tagged
maltose-binding protein (MBP) tagged
Formulario
buffered aqueous solution
selección de bacterias
kanamycin
Origen de replicación
2Micron
pUC (500 copies)
Escisión peptídica
no cleavage
Ubicación de la etiqueta en el péptido
N-terminal
Promotor
Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast
Condiciones de envío
ambient
temp. de almacenamiento
−20°C
Descripción general
This pack provides our entire range of 10 yeast functional tags, including epitope, affinity and solubility tags. Inserting your gene of interest into the MCS will place the tag downstream (at the C terminus of the protein), and the gene will be regulated by the strong constitutive yeast TEF1 promoter. This pack therefore provides a versatile resource for adding functional tags to several plasmids, but also enables you to compare different tags on the same protein, to identify which works best in your experimental system.This plasmid set has been designed to be compatible with a range of cloning techniques. The multiple cloning site contains a range of standard commonly used restriction sites for cloning. Using these sites genes can be inserted using standard cloning methods with DNA ligase. Other methods such as ligase independent cloning (LIC) Gibson Assembly InFusionHD or Seamless GeneArt can also be used and because all of our plasmids are based on the same backbone the same method can be used for cloning into all of our catalogue vectors.
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI. The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site. Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
Transcription Termination: These plasmids contains three alternative transcription terminators for mammalian bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.
Secuencia
Nota de análisis
Otras notas
Información legal
Los componentes del kit también están disponibles por separado
- OGS3456PSF-TEF1-COOH-HIS6 - C-TERMINAL 6 HIS TAG YEAST PLASMID, plasmid vector for molecular cloningSDS
- OGS3458PSF-TEF1-COOH-FLAG® - C-TERMINAL FLAG® TAG YEAST PLASMID, plasmid vector for molecular cloningSDS
- OGS3459PSF-TEF1-COOH-CMYC - C-TERMINAL C-MYC TAG YEAST PLASMID, plasmid vector for molecular cloningSDS
- PSF-TEF1-COOH-HA - C-TERMINAL HA TAG YEAST PLASMID, plasmid vector for molecular cloning
- PSF-TEF1-COOH-GST - C-TERMINAL GST TAG YEAST PLASMID, plasmid vector for molecular cloning
- PSF-TEF1-COOH-MBP - C-TERMINAL MBP TAG YEAST PLASMID, plasmid vector for molecular cloning
Producto relacionado
Código de clase de almacenamiento
12 - Non Combustible Liquids
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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