P5994

Sustrato de fosfatasa

40 mg tablets

• Sinónimos: Fosfato de 4-nitrofenilo disodium salt hexahydrate, pNPP disodium salt hexahydrate
• Fórmula lineal: O₂NC₆H₄OP(O)(ONa)₂ · 6H₂O
• Número CAS: 333338-18-4
• Peso molecular: 371.14
• NACRES: NA.54
• PubChem Substance ID: 24898723
• UNSPSC Code: 12352204
• EC Number: 224-246-5
• MDL number: MFCD00066288

Propiedades

• Quality Segment: 200
• form: tablet
• concentration: 36-44 mg/tablet
• impurities: p-nitrophenol, essentially free
• solubility: H₂O: soluble, clear, colorless to yellow (1 tablet in 8 mL of water.)
• storage temp.: −20°C
• SMILES string: O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
• InChI: 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
• InChI key: KAKKHKRHCKCAGH-UHFFFAOYSA-L

Descripción

General description

Human phosphatase-substrate networks are mapped based on the literature and protein-protein interaction (PPI) database. Based on gene expression data, the possible network of phosphatase-substrate-kinase is predicted.

Application

Sustrato cromógeno para usar en procedimientos ELISA de fosfatasa ácida y la alcalina. Rinde un producto final soluble que es de color amarillo y puede leerse espectrofotométricamente a 405 nm. La reacción de pNPP puede detenerse con NaOH 3 N y leerse a 405 nm.

Phosphatase is a chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. It is been used for the colorimetric detection of CTB fusion proteins[1]. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.

The substrate has been used to determine the alkaline phosphatase (ALP) activity in human osteoblast-like Saos-2 cell lysates. ALP acts as an early marker of osteoblast differentiation that is expressed just before matrix mineralization begins.

Phosphatase substrate has been used as a substrate in alkaline phosphatase assay in serum samples, bone marrow stromal cells and human mesenchymal stem cells (hMSC).[2]

Preparation Note

Contains 40 mg substrate per tablet

Dissolve tablets to the desired concentration in either of the following buffers: 0.1 M glycine, pH 10.4, with 1 mM MgCl₂ and 1 mM ZnCl₂ or 1 M diethanolamine, pH 9.8, with 0.5 mM MgCl₂. Typically, a pNPP concentration of 1 mg/mL is used.

Other Notes

Substrate for the determination of acid and alkaline phosphatases.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)