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P5726

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Phosphatase Inhibitor Cocktail 2

aqueous solution (dark coloration may develop upon storage, which does not affect the activity), for the inhibition of tyrosine protein phosphatases, acid and alkaline phosphatases

Sinónimos:

Protease inhibitor

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.54

product name

Phosphatase Inhibitor Cocktail 2, aqueous solution (dark coloration may develop upon storage, which does not affect the activity)

form

aqueous solution (dark coloration may develop upon storage, which does not affect the activity)

Quality Level

300

shipped in

wet ice

Storage temp.

2-8°C

Categorías relacionadas

General description

Phosphatase Inhibitor Cocktail 2 is a mixture of components designed to inhibit enzymes that modify proteins present in cell extracts, leading to improved yields of intact proteins. It has been optimized and tested for use on various animal tissues and cytosolic extracts.

Application

The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been optimized and tested for tyrosine protein phosphatases, acid and alkaline phosphatases. It has been tested on cell extracts from animal tissues including bovine liver and human placenta, and cytosolic extract of rabbit muscle.

Biochem/physiol Actions

This mixture contains individual components with specific inhibitory properties. Sodium orthovanadate inhibits a number of ATPases, protein tyrosine phosphatases, and other phosphate-transferring enzymes. Sodium molybdate inhibits acid and phosphoprotein phosphatases. Sodium tartrate inhibits acid phosphatases. Imidazole inhibits alkaline phosphatases.

Features and Benefits

This compound is a featured product for Kinase Phosphatase Biology research. Click here to discover more featured Kinase Phosphatase Biology products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Caution

As supplied, this product is stable for two years at the recommended 2-8°C storage temperature.

Preparation Note

This cocktail is supplied as a clear aqueous solution. It has been sterile filtered through a 0.2 μm membrane in bottles that have been aseptically filled. One mL will inhibit phosphatase activities found in the 100,000 x g supernatant from human placenta, bovine liver, rabbit muscle, A431, or Jurkat cell extracts at a protein concentration of approximately 5 mg/mL.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

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Pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 2

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Colin S McCoin et al.
American journal of physiology. Endocrinology and metabolism, 317(2), E298-E311 (2019-05-01)
The impact of sexual dimorphism and mitophagy on hepatic mitochondrial adaptations during the treatment of steatosis with physical activity are largely unknown. Here, we tested if deficiencies in liver-specific peroxisome proliferative activated-receptor-γ coactivator-1α (PGC-1α), a transcriptional coactivator of biogenesis, and
Blanca Majem et al.
Oncogene, 38(32), 6035-6050 (2019-07-07)
Ovarian cancer is the most lethal gynecological malignancy due to the silent nature on its early onset and the rapid acquisition of drug resistance. Histologically heterogeneous, it includes several subtypes with different mutational landscapes, hampering the development of effective targeted
Shira Simonovitch et al.
Journal of Alzheimer's disease : JAD, 70(3), 861-875 (2019-07-16)
This study examined the effects of apolipoprotein E4 (APOE4), the most prevalent genetic risk factor for Alzheimer's disease (AD), on proteins involved in mitochondrial dynamics and autophagy, in the hippocampus of targeted replacement mice. Immunohistochemical measurements revealed that the levels
Uchenna John Unachukwu et al.
Scientific reports, 6, 22392-22392 (2016-03-05)
To replace photoreceptors lost to disease or trauma and restore vision, laboratories around the world are investigating photoreceptor replacement strategies using subretinal transplantation of photoreceptor precursor cells (PPCs) and retinal progenitor cells (RPCs). Significant obstacles to advancement of photoreceptor cell-replacement
G I Lancaster et al.
Nature communications, 7, 10626-10626 (2016-02-04)
Protein kinase R (PKR) has previously been suggested to mediate many of the deleterious consequences of a high-fat diet (HFD). However, previous studies have observed substantial phenotypic variability when examining the metabolic consequences of PKR deletion. Accordingly, herein, we have
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Phosphatase Inhibitor Cocktail

The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.

Discover Bioactive Small Molecules for Kinase Phosphatase Biology

Discover Bioactive Small Molecules for Kinase Phosphatase Biology

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