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P2443

Sigma-Aldrich

Pluronic® F-127

powder, BioReagent, suitable for cell culture

Sinónimos:

Non-ionic copolymer surfactant

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250 G
$69.10
1 KG
$232.00

$69.10

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250 G
$69.10
1 KG
$232.00

About This Item

Fórmula lineal:
(C3H6O·C2H4O)x
Número de CAS:
9003-11-6
MDL number:
MFCD00082049
UNSPSC Code:
12161902
PubChem Substance ID:
24897874
NACRES:
NA.75

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description

Non-ionic
contains 100ppm BHT

Quality Level

200

product line

BioReagent

form

powder

mol wt

~12600 g/mol

technique(s)

cell culture | mammalian: suitable

CMC

950-1000 ppm (~25°C)

transition temp

cloud point >100 °C

HLB

18 - 23

InChI

1S/C3H6O.C2H4O/c1-3-2-4-3;1-2-3-1/h3H,2H2,1H3;1-2H2

InChI key

RVGRUAULSDPKGF-UHFFFAOYSA-N

Categorías relacionadas

General description

Pluronicpluronic® F-127, también conocido como poloxámero 407, es un copolímero no tóxico[1] y no iónico. Contiene óxido de polietileno-óxido de polipropileno dispuesto en una estructura tribloque para formar PEO-PPO-PEO simétrico.[2] Las propiedades tensioactivas de este polímero, derivadas de su naturaleza anfifílica, le permiten interactuar con superficies hidrófobas y membranas biológicas. [3]

Application

Pluronic® F-127 se ha utilizado:

  • como un sistema de administración de un péptido al sitio de la herida para estudiar el efecto del péptido en la cicatrización de las heridas en ratones.[4]
  • como revestimiento superficial para sellos de PDMS (polidimetilsiloxano) para volverlos repelentes a hidrogel.[5]
  • en la preparación de disolución indicadora de calcio de reserva para medir las actividades neuronales.[6]
  • como componente de la disolución salina tamponada con fosfato (PBS), para reducir la adhesión celular y proteica inespecífica a un dispositivo microfluídico basado en PDMS.[7]
  • Este copolímero tensioactivo no iónico es adecuado para utilizar como antiespumante en aplicaciones de cultivo de células de insectos.

Biochem/physiol Actions

Pluronic® F-127 es un gel termorreversible que encuentra su aplicación en diferentes industrias debido a su gran termosensibilidad, biodegradabilidad y biocompatibilidad.[1] Estas propiedades hacen que el polímero sea adecuado para utilizar como transportador versátil de medicamentos. Pluronic® F-127 mejora la eficiencia terapéutica de los medicamentos al aumentar su biodisponibilidad y su termoestabilidad.[8] Además, puede promover la unión celular y la formación de colágeno, lo que facilita la angiogénesis y la regeneración tisular. Debido a su eficacia, Pluronic F-127 se ha utilizado en la encapsulación de células madre mesenquimatosas (MSCs) para ayudar en la regeneración de tejidos con bajos niveles de vascularidad, como los tejidos epiteliales, los tejidos adiposos, los cartílagos, los tendones y los tejidos óseos.[1]

Legal Information

Pluronic is a registered trademark of BASF

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Federico Ceriani et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(46), E7194-E7201 (2016-11-04)
Spatially and temporally coordinated variations of the cytosolic free calcium concentration ([Ca2+]c) play a crucial role in a variety of tissues. In the developing sensory epithelium of the mammalian cochlea, elevation of extracellular adenosine trisphosphate concentration ([ATP]e) triggers [Ca2+]c oscillations
Dual-responsive (pH/temperature) Pluronic F-127 hydrogel drug delivery system for textile-based transdermal therapy
Chatterjee S, et al.
Scientific Reports, 9(1) (2019)
Environmentally responsive injectable materials
Bearat HH and Vernon BL
Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine, 263-297 (2011)
Philippe Marmottant et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(41), 17271-17275 (2009-10-07)
Cell aggregates are a tool for in vitro studies of morphogenesis, cancer invasion, and tissue engineering. They respond to mechanical forces as a complex rather than simple liquid. To change an aggregate's shape, cells have to overcome energy barriers. If
NeuroCa: integrated framework for systematic analysis of spatiotemporal neuronal activity patterns from large-scale optical recording data
Jang MJ and Nam Y
Neurophotonics, 2(3) (2015)
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Local delivery of bioactive molecules using an implantable device can decrease the amount of drug dose required as well as non-target site toxicities compared to oral or systemic drug administration.

Contenido relacionado

Ingeniería de tejidos

En la ingeniería de tejidos se fabrican cultivos de tejidos a partir de armazones, células vivas y moléculas biológicamente activas simulando el microentorno del organismo para reparar o sustituir tejido dañado.

Tissue Engineering

Tissue engineering fabricates tissues cultures from scaffolds, living cells, and biologically active molecules by simulating the microenvironment of the body to repair or replace damaged tissue.

Questions

1–10 of 13 Questions  

  1. Is there a reference for using Pluronic F-127 to reduce cell adhesion in a microfluidic PDMS-based device? Also, does it dissolve in cell culture media in a PDMS-based device, and if it does, what modifications are recommended to maintain its ability to prevent cell adhesion?

    1 answer

    1. There are several representative publications that report the use of Pluronic F-127 to lower cell adhesion on microfluidic PDMS-based devices. These publications may be of interest as a reference:

      Curr Protoc Cell Biol. 2017 Jun 19;75:10.21.1-10.21.8. Microcontact Peeling: A Cell Micropatterning Technique for Circumventing Direct Adsorption of Proteins to Hydrophobic PDMS. PMID: 28627756

      Sci Rep. 2019 May 14;9(1):7377. Simple Surface Modification of Poly(dimethylsiloxane) via Surface Segregating Smart Polymers for Biomicrofluidics. PMID: 31089162

      Lab Chip. 2009 Jun 7;9(11):1500-3. Surface modification of PDMS by gradient-induced migration of embedded Pluronic. PMID: 19458853

      These publications provide information on using Pluronic F-127 to lower cell adhesion on PDMS-based devices. As for its solubility in cell culture media within a PDMS-based device and the recommended modifications to maintain its ability to prevent cell adhesion, it is advised to refer to these publications for detailed insights.

      Helpful?

  2. I was asked about the polydispersity of Pluronic F-127, Product P2443. Could you please inform me?

    1 answer

    1. The polydispersity is not a test specification for this product. The product is an ABA-type copolymer of poly (ethylene oxide) (PEO=A) and poly (propylene oxide) (PPO=B), where the A and B blocks have the following values: A = 101, B = 56. The approximate relative amount of PEO in the poloxamer is ca. 70% and the average molecular weight of the PPO is 4000. The molecular structure is shown below:

      Helpful?

  3. can I know what is the chain length of each PEO and PPO of this F-127?

    1 answer

    1. Pluronic F 127 corresponds to the structure of Kolliphor P 407.
      This product is an ABA-type copolymer of polyethylene oxide (PEO=A) and polypropylene oxide (PPO=B), where the A and B blocks have the following values:
      A = 101
      B = 56

      Helpful?

  4. What is the VOC content of this product?

    1 answer

    1. Volatile Organic Compound (VOC) content is not measured for this product.

      Helpful?

  5. Une fois le Pluronic® F-127 mis en solution (à 10% dans de l'eau par exemple), combien de temps et à quelle température se conserve-t-il ?

    1 answer

    1. The solubility of the product is 50 mg/ml water, at less than 10°C. The stability of the product once diluted has not been tested, in which case it is suggested to prepare the necessary quantity before use.

      Helpful?

  6. What is the best way to sterilise Pluronic F127 dissolved in water so that it is suitable for cell culture?

    1 answer

    1. The solution can be sterilized with a 0.22 uM sterile filter.

      Helpful?

  7. Is corn oil soluble in Pluronic PBS gel?

    1 answer

    1. In general, Pluronic acid products are poor solubilizers of oils. However, they may make a good dispersant or emulsifier for corn oil.

      Helpful?

  8. Is Pluronic® F-127 the same chemical as Poloxamer 407 (16758-250G)? CAS appears to be the same

    1 answer

    1. Yes, Pluronic® F-127 is the same chemical as Poloxamer 407 as stated under Depositor Supplied

      Synonyms on PubChem are available at this link: https://pubchem.ncbi.nlm.nih.gov/compound/24751#section=Depositor-Supplied-Synonyms.
      Kindly review each of the specifications and tested applications for each product before making a purchasing decision.

      Helpful?

  9. What is the molecular weight of Pluronic F-127, Product P2443?

    1 answer

    1. The average molecular weight is 12.6 kDa.

      Helpful?

  10. Why has my solution of Pluronic F-127, Product P2443, gelled?

    1 answer

    1. Pluronic F-127 will form gels above 10 °C when used at concentrations of 18-50%. It will re-liquefy when cooled to below 10 °C. The gel should remain stable during autoclaving.

      Helpful?

1–10 of 13 Questions  

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