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OGS3211

PSF-CMV-PURO-NH2-HIS6 - N-TERMINAL 6 HIS TAG MAMMALIAN PLASMID

Name: PSF-CMV-PURO-NH2-HIS6 - N-TERMINAL 6 HIS TAG MAMMALIAN PLASMID
Brand: SIGMA

plasmid vector for molecular cloning

Sinónimos:

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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Todas las fotos(2)

About This Item

UNSPSC Code:

12352200

NACRES:

NA.85

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pCDFDuet-1 DNA - Novagen

tag

6-His tagged

form

buffered aqueous solution

mol wt

size 6167 bp

bacteria selection

kanamycin

mammalian cells selection

puromycin

origin of replication

pUC (500 copies)

peptide cleavage

no cleavage

peptide tag location

N-terminal

promoter

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

reporter gene

none

shipped in

ambient

storage temp.

−20°C

General description

This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.

About the Peptide Tag:This plasmid contains an n-terminal Hexa-Histidine (6His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHH.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

Sequence

To view sequence information for this product, please visit the product page

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxgene.com

related product

Referencia del producto

Descripción

Precios

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we

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The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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Documentos

PSF-CMV-PURO-NH2-HIS6 - N-TERMINAL 6 HIS TAG MAMMALIAN PLASMID

plasmid vector for molecular cloning

About This Item

Productos recomendados

Propiedades

tag

form

mol wt

bacteria selection

mammalian cells selection

origin of replication

peptide cleavage

peptide tag location

promoter

reporter gene

shipped in

storage temp.

Descripción

General description

Sequence

Analysis Note

Productos relacionados

related product

Información de seguridad

Storage Class

flash_point_f

flash_point_c

Documentación

Certificados de análisis (COA)

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Artículos con revisión científica externa.

Servicio técnico