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MilliporeSigma

N6789

Sigma-Aldrich

Anti-NUCB2 (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti-NEFA, Anti-Nef, Anti-Nesfatin, Anti-Nucleobindin 2, Anti-P54

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~54 kDa

species reactivity

rat, mouse

packaging

antibody small pack of 25 μL

concentration

~1.5 mg/mL

technique(s)

indirect immunofluorescence: 10-20 μg/mL using NIH3T3 cells
western blot: 1.5-3.0 μg/mL using rat adrenal extract (S1 fraction) and mouse testis extract (S1 fraction)

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

General description

Nucleobindin 2 (NUCB2), also known as DNA binding/EF-hand/acidic protein (NEFA), p54, is encoded by the gene mapped to human chromosome 11p15. It belongs to the nucleobindin family of EF-hand Ca2+ - binding proteins. NUCB2 contains multiple functional domains, including a Leu/Ile rich region, a putative nuclear localization signal, a DNA binding domain, two Ca2+ -binding EF-hand motifs and a leucine zipper region. NUCB2 is widely distributed and is localized to the Golgi apparatus. NUCB2 has been shown to interact with both necdin and ARTS-1, through the EF-hand motif.

Specificity

Anti-NUCB2 (C-terminal) specifically recognizes rat and mouse NUCB2.

Application

Anti-NUCB2 (C-terminal) antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Nucleobindin 2 (NUCB2) has been implicated in various functions, including Ca2+ homeostasis, hypothalamic regulation of feeding and tumor necrosis factor (TNF)-receptor-1 (TNFR1)shedding. The NUCB2-ARTS-1 complex is essential for the proteolytic cleavage and release of TNFR1 to the extracellular compartment. NUCB2 has been identified as a novel satiety molecule in the hypothalamus associated with melanocortin signaling in the central nervous system. NUCB2/nesfatin-1 has been shown to induce suppression of food-intake in rats.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Modulation of nucleobindin-1 and nucleobindin-2 by caspases
Valencia CA, et al.
Febs Letters, 582(2), 286-290 (2008)
Candidate genes in patients with autoinflammatory syndrome resembling tumor necrosis factor receptor-associated periodic syndrome without mutations in the TNFRSF1A gene
Borghini S, et al.
The Journal of Rheumatology, 38(7), 1378-1384 (2011)
Peripheral administration of nesfatin-1 reduces food intake in mice: the leptin-independent mechanism
Shimizu H, et al.
Endocrinology, 150(2), 662-671 (2009)
Molecular characterisation and expression analysis of SEREX-defined antigen NUCB2 in gastric epithelium, gastritis and gastric cancer
Kalnina Z, et al.
European Journal of Histochemistry, 53(1), 1378-1384 (2009)
Anthony Ravussin et al.
Cell reports, 24(5), 1085-1092 (2018-08-02)
Inducers of satiety are drug targets for weight loss to mitigate obesity-associated diseases. Nucleobindin-2 (Nucb2) is thought to be post-translationally processed into bioactive nesfatin-1 peptide, which reportedly induces satiety, causes weight loss, and thus improves insulin sensitivity. Here, we show

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