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N6255

Sigma-Aldrich

β-Nicotinamide adenine dinucleotide phosphate–Agarose

saline suspension

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About This Item

MDL number:
UNSPSC Code:
23151817

form

saline suspension

extent of labeling

2-4 μmol per mL

matrix

Cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

ribose hydroxyls

matrix spacer

11 atoms (adipic acid dihydrazide)

storage temp.

2-8°C

Application

NADP-agarose is used for protein chromatography, affinity chromatography and nucleotide/coenzyme resins. NADP-agarose has been used to evaluate macroporous scaffolds for bone tissue engineering for biomedical applications.

Physical form

Suspension in 0.5 M NaCl containing 0.02% thimerosal

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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J A Puértolas et al.
Acta biomaterialia, 7(2), 841-847 (2010-08-17)
There is an acknowledged need for shaping 3-D scaffolds with adequate porosity and mechanical properties for biomedical applications. The mechanical properties under static and cyclic compressive testing of dense and designed porous architecture bioceramic scaffolds based on the biphasic calcium
Sreelatha T Reddy et al.
Protein expression and purification, 26(2), 290-300 (2002-10-31)
Mannose 6-phosphate receptors (MPRs) form essential components of the lysosomal enzyme targeting system by binding newly synthesized acid hydrolases with high (nM) affinity. We report the use of Pichia pastoris as a host to efficiently express the extracytoplasmic ligand-binding domain
D Wyrambik et al.
European journal of biochemistry, 97(2), 503-509 (1979-07-01)
Isoenzyme 2 of cinnamyl-alcohol dehydrogenase from soybean suspension cultures was purified about 3800-fold to apparent homogeneity by an improved purification procedure involving biospecific elution of the enzyme from a NADP+-agarose column. On sodium dodecylsulfate gels the dehydrogenase showed only one
C Andriamampandry et al.
The Biochemical journal, 334 ( Pt 1), 43-50 (1998-08-07)
The gamma-hydroxybutyrate biosynthetic enzyme succinic semialdehyde reductase (SSR) was purified to homogeneity from rat brain. Peptides were generated by tryptic cleavage and sequenced. PCR primers were designed from the amino acid sequences of two of the peptides showing a similarity
M Fisher et al.
Enzyme and microbial technology, 16(4), 281-285 (1994-04-01)
Nonradioactive immunoassays incorporating an element of amplification in their detection system require the use of components that are highly purified. Flavin adenine dinucleotide-3'-phosphate (FADP) is the primary substrate used in such an amplification assay. For incorporation into a simple, single-pot

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