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MCL1

Sigma-Aldrich

Mammalian Cell Lysis Kit

Sinónimos:

Cell Lysis Reagent

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.56

usage

 kit sufficient for 250 extractions (100 mm tissue culture plates)

Quality Level

200

technique(s)

immunoprecipitation (IP): suitable

shipped in

dry ice

storage temp.

−20°C

Categorías relacionadas

Application

The product contains all required reagents for the disintegration of mammalian cells and tissue for subsequent analysis of protein targets. Cellular proteins are stabilized during lysis and are suitable for immunoassays, immunoprecipitation, affinity purification and other functional assays.
Mammalian cell lysis kit was used to test the role of vascular endothelial growth factor (VEGF) receptor-1 and its ligand VEGF-B in motor neuron degeneration.

Features and Benefits

  • The kit can be used to prepare cell lysis buffer and other modified buffers
  • The kit enables the researcher to change the buffer′s components to obtain maximum efficiency for a specific protein

Components

The mammalian cell lysis kit is composed of solutions to be mixed and used for lysis of adherent cells, non-adherent cells, and tissues. It contains buffer, detergents, NaCl and protease inhibitor cocktail solutions.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • P8340Protease inhibitor cocktail 2.5 mLSDS

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Shintaro Nakao et al.
The American journal of pathology, 178(4), 1913-1921 (2011-03-26)
Vascular adhesion protein-1 (VAP-1) contributes to inflammatory and angiogenic diseases, including cancer and age-related macular degeneration. It is expressed in blood vessels and contributes to inflammatory leukocyte recruitment. The cytokines IL-1β and vascular endothelial growth factor A (VEGF-A) modulate angiogenesis
Shintaro Nakao et al.
Blood, 117(3), 1081-1090 (2010-08-14)
Angio- and lymphangiogenesis are inherently related processes. However, how blood and lymphatic vessels regulate each other is unknown. This work introduces a novel mechanism explaining the temporal and spatial relation of blood and lymphatic vessels. Vascular endothelial growth factor-A (VEGF-A)
Shinsuke Yasuda et al.
Journal of immunology (Baltimore, Md. : 1950), 179(7), 4890-4900 (2007-09-20)
Dysregulation of Ras guanyl nucleotide-releasing protein 1 (RasGRP1) in mice results in a systemic lupus erythematosus (SLE)-like disorder. We therefore looked for defective isoforms and/or diminished levels of human RasGRP1 in a cohort of SLE patients. PBMCs were collected from
Molecular imaging reveals elevated VEGFR-2 expression in retinal capillaries in diabetes: a novel biomarker for early diagnosis.
Sun D, Nakao S, Xie F, et al.
Faseb Journal (2014)
Jie Liu et al.
Frontiers in physiology, 6, 69-69 (2015-03-26)
In many non-excitable and excitable cells, store-operated calcium entry (SOCE) represents an additional pathway for calcium entry upon Ca(2+) store depletion. In a previous study, we demonstrated SOCE activity in intact mouse cardiac pacemaker tissue, specifically from sinoatrial node (SAN)
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