MAK518

ADP Assay Kit

sufficient for 100 fluorometric tests

• Sinónimos: ADP Detection Kit
• Código UNSPSC: 12161503
• NACRES: NA.84

Propiedades

• Nivel de calidad: 100
• método de detección: fluorometric
• enfermedades relevantes: hematological disorder
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Adenosine diphosphate (ADP) is the product of ATP dephosphorylation by ATPases. ADP can be converted back to ATP by ATP synthases. ADP levels regulate several enzymes involved in intermediary metabolism. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, since these assays require measurement of ATP in the sample before conversion of ADP to ATP, if the nascent ATP concentration is significantly higher than the ADP concentration, the ATP signal will drown out the ADP signal.

Características y beneficios

• As low as 0.1 µM ADP can be quantified.
• Compatible with high-throughput handling systems.

Idoneidad

The kit is suitable for ADP determination in cells and other biological samples.

Principio

The ADP Assay Kit provides a convenient fluorometric means to measure ADP level even in the presence of ATP. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate is then quantified by a fluorometric method at λEx = 530 nm/λEm = 590 nm.

Información de seguridad

• Pictogramas: GHS05,GHS07,GHS09
• Palabra de señalización: Danger
• Frases de peligro: H290,H314,H335,H411
• Consejos de prudencia: P234 - P261 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Clasificaciones de peligro: Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 8A - Combustible corrosive hazardous materials
• Clase de riesgo para el agua (WGK): WGK 2