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MAK263

Sigma-Aldrich

Glucose Colorimetric/Fluorometric Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric or fluorometric tests

aplicaciones

cosmetics
food and beverages

método de detección

colorimetric
fluorometric

enfermedades relevantes

endocrinological disorders, diabetes

temp. de almacenamiento

−20°C

Descripción general

Glucose is a primary energy source that naturally occurs in its free state in fruits and other plant parts. Abnormal glucose levels have been associated with several metabolic dysfunctions such as hypoglycemia, hyperglycemia, and diabetes mellitus. Measurements of glucose levels in tissues and body fluids (such as blood and urine) are often used for the diagnosis of glucose– related disorders. Glucose levels are also monitored to check the efficacy of therapeutics such as insulin and sulphonylureas in type 2 diabetics.

Características y beneficios

Compatible with high-throughput handling systems.

Idoneidad

Suitable for the determination of glucose concentrations in various biological samples, including serum, plasma, food, or growth medium. This kit is also suitable for monitoring glucose levels during fermentation and glucose feeding in protein expression processes.

Principio

Glucose is oxidized to generate a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the amount of glucose present. The kit is able to detect 1-10,000 μM of glucose in various samples.

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Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H334,H412

Consejos de prudencia

P261 - P273 - P284 - P501

Clasificaciones de peligro

Aquatic Chronic 3 - Resp. Sens. 1

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
8J20K06060
8H05K06060
5K30K06060
J20K06060
8F22K06060

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Daniela Scribano et al.
Molecules (Basel, Switzerland), 25(2) (2020-01-17)
Urinary tract infections (UTIs) are mainly caused by uropathogenic Escherichia coli (UPEC). Acute and recurrent UTIs are commonly treated with antibiotics, the efficacy of which is limited by the emergence of antibiotic resistant strains. The natural sugar d-mannose is considered
Mike Boger et al.
Frontiers in cell and developmental biology, 10, 918529-918529 (2022-07-26)
The ELMO protein family consists of the homologues ELMO1, ELMO2 and ELMO3. Several studies have shown that the individual ELMO proteins are involved in a variety of cellular and developmental processes. However, it has poorly been understood whether the Elmo
Gemma K Kinsella et al.
International journal of molecular sciences, 22(19) (2021-10-14)
GPR21 is a constitutively active, orphan, G-protein-coupled receptor, with in vivo studies suggesting its involvement in the modulation of insulin sensitivity. However, its precise contribution is not fully understood. As the liver is both a major target of insulin signalling
Ryan K Perkins et al.
Nutrients, 11(3) (2019-03-03)
The purpose of this investigation was to evaluate the effects of experimental hyperglycemia on oxidative damage (OX), advanced glycation end products (AGEs), and the receptor for AGEs (RAGE) through an in vivo approach. Obese subjects (n = 10; 31.2 ±
Ye Du et al.
Frontiers in oncology, 10, 580176-580176 (2021-01-05)
Hypoxia is an important environmental factor and has been correlated with tumor progression, treatment resistance and poor prognosis in many solid tumors, including triple-negative breast cancer (TNBC). Emerging evidence suggests that long noncoding RNA (lncRNA) functions as a critical regulator
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Questions

1–2 of 2 Questions  

  1. Can this kit be used on solid tissue samples? If so, how should the tissue be homogenized?

    1 answer

    1. This kit can be used for tissue samples. To homogenize, please proceed as follows:
      Homogenize the tissue sample in assay buffer using the following protocol:

      Start with approximately 10 mg of tissue (the amount may need to be optimized), wash in cold PBS and resuspend in ~100 uL of assay buffer provided with the kit.
      Homogenize using a Dounce homogenizer or a pestle, on ice to keep the sample cold (10-15 passes).
      Centrifuge at 4°C in a cold microcentrifuge to remove insoluble.
      Collect the supernatant and keep it on ice throughout sample preparation.

      Use a few different volumes of this supernatant (between 2 and 50 uL) and bring them up to a total volume of 50 µl/well with Glucose Assay Buffer in a 96-well plate, as recommended for a typical liquid sample.
      The reason for trying different volumes of samples is to ensure the readings are within the standard curve range.
      Then proceed with the 'Assay Reaction' step.

      Please see the datasheet below for the assay reaction step:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/275/602/mak263bul.pdf

      Helpful?

  2. We require a protocol for #MAK263 for my customer

    1 answer

    1. Please see the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/275/602/mak263bul.pdf

      Helpful?

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