This information is proprietary and cannot be disclosed.
MAK016
Kit de análisis del glucógeno
sufficient for 100 colorimetric or fluorometric tests
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Código UNSPSC:
12161503
NACRES:
NA.84
Productos recomendados
uso
sufficient for 100 colorimetric or fluorometric tests
método de detección
colorimetric
fluorometric
temp. de almacenamiento
−20°C
Descripción general
El glucógeno es un polímero ramificado de glucosa que constituye la principal molécula de almacenamiento de energía a corto plazo en animales. El glucógeno se sintetiza principalmente en el hígado y el tejido muscular, donde puede constituir hasta un 10% del peso del hígado y un 1-2% del peso del tejido muscular. Mientras que el glucógeno muscular suele utilizarse localmente, el glucógeno hepático sirve como un importante amortiguador para regular las concentraciones sanguíneas de glucosa. El metabolismo del glucógeno está mal regulado en la diabetes y en las enfermedades de almacenamiento del glucógeno debido a errores innatos del metabolismo.
Aplicación
Idoneidad
Adecuado para determinar la concentración de glucógeno en diversos tejidos, como el hígado, y en cultivo celular (células adherentes o en suspensión).
Principio
La concentración de glucógeno viene determinada por un análisis enzimático acoplado, que da lugar a un producto colorimétrico (570 nm)o fluorométrico (λex = 535/λem = 587 nm), proporcional al glucógeno presente.
sustituido por
Referencia del producto
Descripción
Precios
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1 - Skin Sens. 1
Código de clase de almacenamiento
10 - Combustible liquids
Punto de inflamabilidad (°F)
188.6 °F - closed cup
Punto de inflamabilidad (°C)
87 °C - closed cup
Elija entre una de las versiones más recientes:
Certificados de análisis (COA)
Lot/Batch Number
¿No ve la versión correcta?
Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Madhuri S Salker et al.
Scientific reports, 7(1), 12612-12612 (2017-10-05)
Embryo implantation requires a hospitable uterine environment. A key metabolic change that occurs during the peri-implantation period, and throughout early pregnancy, is the rise in endometrial glycogen content. Glycogen accumulation requires prior cellular uptake of glucose. Here we show that
Insulin Signaling in Bupivacaine-induced Cardiac ToxicitySensitization during Recovery and Potentiation by Lipid Emulsion
Fettiplace M R, et al.
Anesthesiology, 124(2), 428-\442-428-\442 (2016)
Cassius E O Coombs et al.
Meat science, 134, 86-97 (2017-08-05)
This study evaluated the effect of chilled followed by frozen storage on lamb quality and safety parameters. Experimental (n=360) M. longissimus lumborum (LL) were randomly sampled from the boning room of a commercial Australian abattoir, at 24 h post-mortem, and
Ultrasonic assessment of exercise-induced change in skeletal muscle glycogen content.
Nieman D C, et al.
BMC sports science, medicine & rehabilitation., 7(1), 9-9 (2015)
Ni Zeng et al.
PloS one, 15(4), e0230044-e0230044 (2020-04-03)
LEFTY2 (endometrial bleeding associated factor; EBAF or LEFTYA), a cytokine released shortly before menstrual bleeding, is a negative regulator of cell proliferation and tumour growth. LEFTY2 down-regulates Na+/H+ exchanger activity with subsequent inhibition of glycolytic flux and lactate production in
-
What is the polymer size of glycogen used in the standard of the MAK016-1KT Glycogen Assay Kit?
1 answer-
Helpful?
-
-
Is it permissible to proceed with boiling the liver samples homogenized in NP40 Substitute assay regent, even though the protocol states that "Tissue (10 mg) can be homogenized in 100 uL of water on ice. Boil homogenates for 5 minutes to inactivate enzymes"?
1 answer-
It is not certain whether the samples prepared in the NP40 Substitute assay reagent will be compatible with this kit. However, one can proceed to the next step by boiling the homogenates for 10 minutes to inactivate enzymes. Following that, the mixture should be centrifuged at 18000 rpm for 10 minutes to remove insoluble material. Collect the supernatant for the assay.
Helpful?
-
-
What could be the reason for the varying cloudiness levels in dilutions of INS-1 beta cell lysate when using the glycogen assay kit (cat #MAK016)? The solution became cloudy after dilution, with different dilutions performed: 1:2, 1:5, and 1:10. The 1:2 dilution appeared cloudier than the 1:5 and 1:10 dilutions, and the 1:5 dilution was cloudier than the 1:10 dilution. The cell lysate was clear after centrifugation before adding it to the hydrolysis buffer.
1 answer-
The observed precipitation may be due to the detergent in the assay buffer when added to the sample. To prevent this, it is recommended to bring the assay buffer to room temperature before adding it. Dilution has shown to reduce turbidity, so using the dilution with the least turbidity is advisable. Testing the performance of this dilution with the standard curve would be beneficial. Considering the high acidity of the hydrolysis buffer, it is uncertain if the pH changes upon adding the lysate, potentially causing precipitation. Testing the pH of the lysate using a pH strip and adjusting it to around 4.5 with HCl, if necessary, while keeping in mind the confirmed pH of the cell lysate is around 7, can be done.
Helpful?
-
-
Is it possible to know information about compatibility with extreme pH, rather than with salt buffers etc...?
1 answer-
The kit is tested with cell culture supernatant, biological fluids, tissue, and urine. It has not been tested with a sample that has an extreme pH. It is not known how that would affect the assay performance, as it has not been tested. However, the assay does work for urine which can have a wide pH range from pH 5-8.
In the protocol, glycogen is extracted using first KOH and then further extracted using HCl. The precipitate is then dissolved in Development Buffer/Hydrolysis Buffer for analysis which is buffered for the assay.
If the pH is already very basic this may be fine as it could be adjusted to the correct pH but if it is very acidic then the extraction may be difficult since the first step is to raise the pH. The buffers in the kit are also pH dependent as well so downstream steps require a specific pH.
Helpful?
-
-
We are looking for a Colorimetric/Fluorometric assay to determine glycogen content in various mouse tissues samples. We came across this kit and would like to know how much sample is required for this assay (volume/well).
1 answer-
The minimum tissue requirement is 10mg in 200 uL. After homogenization and cleanup, a range of 2-50 uL of the sample will be added to each well. For unknown samples it is advised to test several sample dilutions to ensure readings are within linear range. Please see the link below for the Product Information Sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/157/372/mak016bul.pdfHelpful?
-
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico
