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M8042

Sigma-Aldrich

Medio esencial mínimo Eagle

Alpha Modification, with nucleosides and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Sinónimos:

MEM

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500 ML
$64.70
6 X 500 ML
$331.00
24 X 500 ML
$1,130.00

$64.70

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500 ML
$64.70
6 X 500 ML
$331.00
24 X 500 ML
$1,130.00

About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

$64.70

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Nombre del producto

Medio esencial mínimo Eagle, Alpha Modification, with ribonucleosides, deoxyribonucleosides and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

esterilidad

sterile-filtered

Formulario

liquid

técnicas

cell culture | mammalian: suitable

impurezas

endotoxin, tested

componentes

Hanks’ salts (2% CO2): no
glucose: yes
sodium pyruvate: yes
phenol red: yes
HEPES: no
L-glutamine: no
stable glutamine: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.[1]This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Often used in cytogenetic analysis when supplemented with fetal bovine serum (FBS).

Aplicación

Minimum Essential Medium Eagle has been used for the isolation of mesenchymal stem cell, adipose-derived stem cell,[2] and osteoblast.[3]
Minimum Essential Medium Eagle is used for primary osteoblast cultures[4] and for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.[5]

Acciones bioquímicas o fisiológicas

MEM usually autoclaved with 0.5% bactopeptone at 15 psi for 20 min, cooled and then neutralized with NaHCO3, consistently supported good cell growth. A similar result was obtained when the MEM and bactopeptone were autoclaved separately. The cells grow, initially, as a monolayer and subsequently become a stationary suspension.[6]

Reconstitución

Supplement with 0.292 g/L L-glutamine

suplemento

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también adquirido normalmente con este producto

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Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
RNBL9393
RNBL0710
RNBK3712
RNBK2051
RNBJ4241

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A previously functional tetracycline-regulated transactivator fails to target gene expression to the bone
Schmidt E and Eriksson M
BMC Research Notes, 4(1), 282-282 (2011)
Eva Schmidt et al.
BMC research notes, 4, 282-282 (2011-08-13)
The tetracycline-controlled transactivator system is a powerful tool to control gene expression in vitro and to generate consistent and conditional transgenic in vivo model organisms. It has been widely used to study gene function and to explore pathological mechanisms involved
L Keay
Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01)
The growth of L-60TM cells (a suspension culture adapted L-cell) on media composed of MEM (minimum essential medium (Eagle)) and bactopeptone autoclaved together or separately under a variety of conditions has veen determined. It has been found that MEM autoclaved
Isolation of adult stem cells and their differentiation to Schwann cells
Progenitor Cells, 47-57 (2012)
Janhavi Sharma et al.
Journal of immunological methods, 351(1-2), 30-35 (2009-10-06)
One of the initial steps in the inflammatory process involves the adherence and transmigration of circulating polymorphonuclear leukocytes (PMN) across the endothelial cell monolayer. One of the main constituents of the neutrophil phagosome that contributes to bacterial killing is myeloperoxidase
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MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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