M7933

Anti-phospho-MAP Kinase Kinase 3/6 (pSer^189/207) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

• UNSPSC Code: 12352203
• NACRES: NA.44
• MDL number: MFCD02097161
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IP, WB CL
• Citations: 6

Propiedades

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• species reactivity: human, rat, mouse
• technique(s): immunoprecipitation (IP): 1:250, western blot (chemiluminescent): 1:1,000 using anisomycin-treated NIH-3T3 cell extract
• UniProt accession no.: P46734
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pSer189/pSer207)
• Gene Information: human ... MAP2K3(5606) ; mouse ... Map2k3(26397) ; rat ... Map2k3(303200)

Descripción

General description

Activation of MAPK signalling requires the phosphorylation of upstream MAPK kinases or MKKs. MKK3, MKK6 are activate the p38 MAPK isoforms, p38α, p38δ, with the probable involvement of MKK4. MEKKs do not activate the ERK or SAPK/JNK MAPK signalling. MEKK3/6 also mediate the stimulation of pro-α1(l) collagen by TGF-β1 signalling. TGF-β1 signaling stimulates VEGF via activation of p38α and p38β by MEKK3, in murine mesangial cells. Both MEKK3 and MEKK6 are activated in response to cellular stress and inflammatory cytokines. The phosphorylation sites are Ser189 and Thr193 for MKK3 and Ser²⁰⁷ and Thr²¹¹ for MKK6.
Anti-phospho-MAP Kinase Kinase 3/6 (MKK3/6) (phosphoserine 189/207) detects MKK3/MKK6 only when phosphorylated at Ser189/207. It does not cross-react with the corresponding phosphorylated MEK, MEK2, and MKK4/Sek1.

Immunogen

synthetic phospho-Ser¹⁸⁹ peptide corresponding to residues around Ser^189/207 of human MKK3.

Application

Anti-phospho-MAP Kinase Kinase 3/6 (MKK3/6) (phosphoserine 189/207) may be used at a working dilution of 1:1000 for detection by immunoblotting in NIH-3T3 cells treated with anisomycin. The antibody may be used in immunoblotting application in rat hepatoma Fao and human hepatoma HepG2 cells. The antibody is also suitable for immunoprecipitation at a working dilution of 1:250.

Biochem/physiol Actions

The antibody detects MKK3/6 only when phosphorylated at Ser189/207. It does not react with non-phosphorylated MKK3 or MKK6 or phosphorylated MKK4/SEK1, MEK or MEK-2.

Physical form

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.