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M3769

Sigma-Aldrich

Medium 199

Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture

Sinónimos:

M199 Medium, TCM 199

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10 X 1 L
$66.76
50 L
$197.89

$66.76

Precio de catálogo$74.30Ahorre 10 %
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Disponible para envío el08 de abril de 2025Detalles

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10 X 1 L
$66.76
50 L
$197.89

About This Item

Código UNSPSC:
12352207
NACRES:
NA.71

$66.76

Precio de catálogo$74.30Ahorre 10 %
Web-Only Promotion

Disponible para envío el08 de abril de 2025Detalles

Solicitar un pedido a granel

Nivel de calidad

500

Formulario

powder

técnicas

cell culture | mammalian: suitable

componentes

L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Aplicación

Medium 199 has been used:
  • to culture the primary human umbilical vein endothelial cells (HUVECs) for use in cell viability assay
  • to flush the uterine horn of gilts to recover day 12 conceptuses from uteri
  • to culture the peritoneal cells and immortalized human mesothelial cells (MeT-5A and ATCC-CRL-9444)

Cantidad

Formulated to contain 9.4 grams of powder per liter of medium.

Reconstitución

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

suplemento

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Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000436546
0000436546
0000388915
0000388915
0000321888

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Artículos

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Protocolos

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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