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M3769

Medium 199

Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture

Sinónimos:

M199 Medium, TCM 199

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Tamaño de envaseSKUDisponibilidadPrecio
10 x 1 L

Disponible para enviar HOYdesdeMILWAUKEE

$79.60
50 L

Disponible para enviar HOYdesdeMILWAUKEE

$275.00

Acerca de este artículo

NACRES:
NA.71
UNSPSC Code:
12352207

$79.60

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Quality Segment

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:
  • to culture the primary human umbilical vein endothelial cells (HUVECs) for use in cell viability assay
  • to flush the uterine horn of gilts to recover day 12 conceptuses from uteri
  • to culture the peritoneal cells and immortalized human mesothelial cells (MeT-5A and ATCC-CRL-9444)

Preparation Note

Formulated to contain 9.4 grams of powder per liter of medium.
Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

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Este artículo
M2520M2154M0393
Sigma-Aldrich

Sigma-Aldrich

M3769

Medium 199

Sigma-Aldrich

Sigma-Aldrich

M2520

Medium 199

Medium 199 With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

M2154

Medium 199

Medium 199 With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

M0393

Medium 199

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

powder

form

powder

form

liquid

form

powder

components

L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: no

components

L-glutamine: yes
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
sodium pyruvate: no
HEPES: 25 mM

components

sodium pyruvate: no
L-glutamine: no
NaHCO3: yes
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes

components

HEPES: no
Hanks’ salts (2% CO2): yes
sodium pyruvate: no
NaHCO3: no
L-glutamine: yes
phenol red: yes

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

500

Quality Level

500

Quality Level

400

Quality Level

500

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Clase de almacenamiento

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000540073
0000540073
0000437175
0000437175
0000436546

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Protocolos

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Artículos

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Luciana de Lima Ferreira et al.
PLoS neglected tropical diseases, 14(12), e0009015-e0009015 (2020-12-29)
Trypanosoma rangeli is a non-pathogenic protozoan parasite that infects mammals, including humans, in Chagas disease-endemic areas of South and Central America. The parasite is transmitted to a mammalian host when an infected triatomine injects metacyclic trypomastigotes into the host's skin
Diane G Edmondson et al.
mBio, 12(1) (2021-02-25)
The bacterium that causes syphilis, Treponema pallidum subsp. pallidum, has now been cultured in vitro continuously for periods exceeding 3 years using a system consisting of coculture with Sf1Ep rabbit epithelial cells in TpCM-2 medium and a low-oxygen environment. In
J L N Barratt et al.
Parasitology, 137(13), 1867-1878 (2010-07-09)
Dientamoeba fragilis is a pathogen of the human gastrointestinal tract that is a common cause of diarrhoea. A paucity of knowledge on the in vitro cultivation and cryopreservation of Dientamoeba has meant that few studies have been conducted to investigate
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SKUGTIN
M3769-1L04061837473548
M3769-50L04061834050568
M3769-10X1L04061834050551

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