Yes, this product can be dissolved in water at 10 mg/mL. Under these conditions, the product is expected to form a clear, red-brown solution. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/294/482/m0630pis.pdf
M0630
Myoglobin from equine skeletal muscle
95-100%, essentially salt-free, lyophilized powder
Sinónimos:
Myoglobin from horse skeletal muscle
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
250 mg | Envío estimado HOYdesdeMILWAUKEE | $112.00 |
1 g | Envío estimado HOYdesdeMILWAUKEE | $362.00 |
5 g | Envío estimado HOYdesdeMILWAUKEE | $1,280.00 |
10 g | Envío estimado HOYdesdeMILWAUKEE | $2,180.00 |
Acerca de este artículo
Form:
essentially salt-free, lyophilized powder
Assay:
95-100%
Biological source:
equine skeletal muscle
Mol wt:
~17 kDa(lit.)
Servicio técnico
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Permítanos ayudarlebiological source
equine skeletal muscle
Quality Segment
assay
95-100%
form
essentially salt-free, lyophilized powder
mol wt
~17 kDa(lit.)
Iron content
0.25-0.32%
technique(s)
mass spectrometry (MS): suitable
solubility
H2O: soluble 10 mg/mL
UniProt accession no.
storage temp.
−20°C
Gene Information
horse ... MB(100054434)
Application
Biochem/physiol Actions
Myoglobin from horse skeletal muscle is a single chain heme protein containing 153 amino acid residues. It possesses no disulfide bridges or free -SH groups. Myoglobin contains 8 variously sized right-handed helical regions, joined by non-ordered or random coil regions.
Myoglobin is critical to skeletal muscle O2 supply at near-maximum oxygen demand, and prevents anoxia by maintaining PO2 above levels needed to support mitochondrial function.
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Este artículo | |||
|---|---|---|---|
| description 95-100%, essentially salt-free, lyophilized powder | description ≥90% (SDS-PAGE), essentially salt-free, lyophilized powder | description Isoelectric focusing marker, pI (1) 6.8, (2) 7.2 | description culture supernatant, clone 15F4, Chemicon® |
| biological source equine skeletal muscle | biological source equine heart | biological source - | biological source mouse |
| assay 95-100% | assay ≥90% (SDS-PAGE) | assay - | assay - |
| technique(s) mass spectrometry (MS): suitable | technique(s) MALDI-MS: suitable | technique(s) - | technique(s) immunohistochemistry: suitable, western blot: suitable |
| form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form powder | form - |
| mol wt ~17 kDa(lit.) | mol wt - | mol wt - | mol wt - |
| UniProt accession no. | UniProt accession no. | UniProt accession no. - | UniProt accession no. |
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Clase de almacenamiento
11 - Combustible Solids
¿Qué está pasando?
WGK 3
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
EPI (equipos de protección individual)
Eyeshields, Gloves, type N95 (US)
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Is the correct way to prepare a solution of this reagent 10mg/mL of water?
1 answer-
Helpful?
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Do you know the iron content in total for this product (1g)?
1 answer-
The iron content for this product ranges from 0.25% to 0.32%. The specific iron content is lot-dependent and can be found in the Certificate of Analysis.
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Can you advise what the % level of hemoglobin (if any will be present in this product)? Or can this be treated as Hb contaminant free.
1 answer-
While this product is not specifically tested for hemoglobin, it is purified by Sephadex gel filtration. Given the molecular weight differences between these two compounds, the presence of hemoglobin is highly unlikely.
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Can you share the amino acid sequence of this protein? M0630
1 answer-
This product is a native protein that has not been modified during the extraction and purification process. The sequence of this product is published information. Please refer to the information in the UniProt database: https://www.uniprot.org/uniprotkb/P68082/entry
Helpful?
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Is this myoglobin detectable using immunoenzymatic assay?
1 answer-
Unfortunately, none of the equine myoglobin products have been tested for suitability in ELISA. Performance of these products in immunoenzymatic assays cannot be validated.
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