L9639

Ricin B chain from Ricinus communis (castor bean)

buffered aqueous solution

• Número de CAS: 96638-29-8
• MDL number: MFCD00166673
• UNSPSC Code: 12352200

Propiedades

• form: buffered aqueous solution
• storage temp.: 2-8°C

Descripción

Biochem/physiol Actions

Ricin B chain has an affinity for galactose and saccharides containing nonreducing terminal β-galactosyl units. This affinity allows the B chain to bind to cell surface glycoconjugates. In the intact ricin toxin, the surface binding by the B chain allows the A chain to enter into the cell. The saccharide binding capability of ricin B chain is independent of the presence of the A chain.

Other Notes

Ricinus communis toxin (also named RCA₆₀, RCA_II, Ricin D, or RCL III) is a highly toxic protein, mol. wt. ~60 kDa, composed of two chains, A and B, connected by a single disulfide bridge.

Quality

Electrophoretically pure (SDS-PAGE)

Physical form

Solution in 10 mM phosphate, pH 6.5, containing 0.15 M NaCl, 10 mM galactose, 0.5 mM dithioerythritol and 0.02% sodium azide

Preparation Note

Highly purified by affinity chromatography

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 7.2, of a 1 mg protein per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25 °C.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable