There is no specific protocol associated with this product, however our GenElute Bacterial Genomic DNA kits ( NA2100, NA2110, NA2120) include a lysis protocol specific to Staphylococcus species that may be helpful. In this protocol, Lysostaphin is used at 200 units per ml in combination with mutanolysin and a lysis buffer included in the kit. See the link below, page 6 to review this information.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/256/042/na2100bul.pdf
L9043
Lysostaphin from Staphylococcus simulans
recombinant, expressed in E. coli, lyophilized powder
Sinónimos:
Glycyl-glycine Endopeptidase
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5 MG
$377.00
About This Item
Número de CAS:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
Productos recomendados
origen biológico
bacterial
recombinante
expressed in E. coli
Ensayo
≥90% (HPLC)
Formulario
lyophilized powder
actividad específica
≥3000 unit/mg solid
técnicas
molecular cloning: suitable
idoneidad
suitable for sequence analysis test
aplicaciones
life science and biopharma
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
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Descripción general
Lysostaphin, a zinc metalloenzyme, is mainly secreted as a proenzyme. It acts as an anti-staphylococcal agent and is highly stable at pH 4 and 5°C.[1]
Aplicación
Lysostaphin from Staphylococcus simulans has been used in a study to assess molecular cloning, sequencing, and expression of lytM, a unique autolytic gene of Staphylococcus aureus. [2] It has also been used in a study to investigate the sequence analysis of a Staphylococcus aureus gene encoding a peptidoglycan hydrolase activity. [3]
Acciones bioquímicas o fisiológicas
A protein complex with highly specific lytic activity against Staphylococcus species, including Staphylococcus aureus.
Lysostaphin possesses a lytic action against Staphylococcus aureus, which is used to treat antibiotic-resistant staphylococcal infections. It has the properties of enzymes, such as glycylglycine endopeptidase, endo-β-N-acetyl glucosamidase and N-acetyl muramyl-L-alanine amidase. Lysostaphin is also used as a preservative in food industry and in clinical labs for rapid screening.[1]
The antimicrobial activity of lysostaphin, a metalloendopeptidase expressed by Staphylococcus simulans, is enhanced by binding to the C′ terminus of the membrane-associated Trap protein of Staphylococcus aureus. [4]
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Certificados de análisis (COA)
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X Wang et al.
Gene, 102(1), 105-109 (1991-06-15)
The nucleotide (nt) sequence of a 2.0-kb NheI-XbaI DNA fragment containing a peptidoglycan hydrolase-encoding gene, lytA, tentatively identified as encoding an N-acetylmuramyl-L-alanine amidase, from Staphylococcus aureus, was determined. The nt sequencing revealed an open reading frame (ORF) of 1443 bp
L Ramadurai et al.
Journal of bacteriology, 179(11), 3625-3631 (1997-06-01)
A gene encoding an autolytic activity was identified in an autolysis-deficient mutant (Lyt-) of Staphylococcus aureus which produces only a single band in autolytic-activity gels (N. Mani, P. Tobin, and R. K. Jayaswal, J. Bacteriol. 175:1493-1499, 1993). An open reading
C-terminus of TRAP in Staphylococcus can enhance the activity of lysozyme and lysostaphin.
Yang, G.
Acta Biochimica et Biophysica Hungarica, 40, 452-458 (2008)
Lysostaphin: an antistaphylococcal agent
Kumar J K
Applied Microbiology and Biotechnology, 80(4), 555-561 (2008)
Grégory Francius et al.
Journal of bacteriology, 190(24), 7904-7909 (2008-10-07)
The advent of Staphylococcus aureus strains that are resistant to virtually all antibiotics has increased the need for new antistaphylococcal agents. An example of such a potential therapeutic is lysostaphin, an enzyme that specifically cleaves the S. aureus peptidoglycan, thereby
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Is there a standard protocol from the manufacturer on how to lyse Staphylococcus aureus cells? What concentration, temperature, time ...
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How should a stock solution of the Lysostaphin from Staphylococcus simulans enzyme, product L9043, be prepared?
1 answer-
A stock solution of this recombinant, Lysostaphin from Staphylococcus simulans, may be prepared by reconstituting it at 10 mg/ml in 20 mM sodium acetate buffer, pH 4.5 and storing it aseptically at 4°C. in a polypropylene container.
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