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L8262

Lectin from Ulex europaeus (gorse, furze)

biotin conjugate, lyophilized powder

Sinónimos:

Ulex europaeus agglutinin, UEA-I

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Acerca de este artículo

NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
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conjugate

biotin conjugate

Quality Segment

form

lyophilized powder

potency

<50 μg/mL agglutination activity

composition

Protein, ~85% E1%/280

extent of labeling

~3 mol biotin per mol protein

storage temp.

2-8°C

Application

Lectin from Ulex europaeus (gorse, furze) has been used:
  • as a Paneth cells marker in immunohistochemistry at 1:1,000 dilution [1]
  • in double staining for endothelial cells for quantitation of blood vessels[2]
  • in histochemical staining of bronchial epithelial cells[3]

Biochem/physiol Actions

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Packaging

Package size based on protein content.

Physical form

Contains sodium citrate

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Other Notes

Conjugates are prepared from affinity purified lectin.

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Este artículo
L9006L5505L9640
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

potency

<50 μg/mL agglutination activity

potency

<5 μg/mL agglutination activity

potency

<8 μg/mL agglutination activity

potency

<20 μg/mL agglutination activity

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

conjugate

biotin conjugate

conjugate

FITC conjugate

conjugate

-

conjugate

-

extent of labeling

~3 mol biotin per mol protein

extent of labeling

2-5 mol FITC per mol protein

extent of labeling

-

extent of labeling

-


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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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