I5020

Anti-Interleukin 10 antibody produced in goat

IgG fraction of antiserum, lyophilized powder

• Sinónimos: Anti-IL-10
• Número CE: 202-739-6
• Número MDL: MFCD00212980
• Código UNSPSC: 51111800
• NACRES: NA.41

Propiedades

• origen biológico: goat
• conjugado: unconjugated
• forma del anticuerpo: IgG fraction of antiserum
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: lyophilized powder
• reactividad de especies: human
• técnicas: neutralization: suitable
• Nº de acceso UniProt: P22301
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... IL10(3586)

Descripción

Descripción general

Interleukin 10 is located on human chromosome 1q32.1.

Interleukin-10 (IL-10) is a pleotropic cytokine with anti-inflammatory and immunosuppressive functions. It is the key cytokine that regulates the host inflammatory responses to bacterial, fungal and viral pathogens. The production of IL-10 is mediated by signalling of IL-6, IL-12 and TGF-β. IL-10 is secreted by a variety of cells including T cells, B cells, dendritic cells, eosinophils, natural killer cells and mast cells. The effects of IL-10 are mediated by binding to the IL-10R, consisting of α and β subunits. The signalling through IL-10R activates Jak1, Tyk2 protein kinases and JAK/STAT3 pathways. IL-10 is involved in suppression of innate immunity to kill pathogens and inhibits the release of reactive oxygen and nitrogen species from activated macrophages. Regulation of IL-10 production is important in infections, inflammatory responses in autoimmune encephalomyelitis, endotoxic shock and chronic enterocolitis
Anti-interleukin-10 antibody is specific for human IL-10. The antibody shows ~7% cross reactivity with recombinant mouse IL-10 in ELISA and immunoblotting.

Especificidad

The antibody neutralizes the biological activity of recombinant human IL-10, but not recombinant mouse IL-10.

Inmunógeno

recombinant human IL-10 expressed in Sf 21 insect cells.

Aplicación

Anti-interleukin 10 antibody produced in goat has been used to measure the anti-allodynic effect of IL-10 in the neuropathic pain rat model.

Anti-interleukin-10 antibody may be used for immunoblotting at a working concentration of 1 μg/ml. For ELISA a working concentration of 0.25 μg/ml is recommended. The antibody is suitable for neutralization reactions. This antibody has also been used for immunohistochemistry of cervicovaginal epithelial cells of rabbit

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Acciones bioquímicas o fisiológicas

Interleukin 10 (IL10) helps to induce anti-tumor immune response. It controls tumor tolerance in non-small cell lung cancer. IL10 prevents the synthesis of pro-inflammatory cytokines by activating the JAK/STAT (janus kinase/signal transducers and activators of transcription) signalling pathway.

Forma física

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Nota de preparación

Purified using protein G.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)