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H5527

Sigma-Aldrich

Hygromycin B solution from Streptomyces hygroscopicus

≥60% (HPLC), 45-60 mg/mL in H2O, γ-irradiated

Sinónimos:

Hygromycin b, Hygrovetine

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About This Item

Fórmula empírica (notación de Hill):
C20H37N3O13
Número de CAS:
31282-04-9
Peso molecular:
527.52
Beilstein:
6755837
Número MDL:
MFCD06795479
Código UNSPSC:
51102829
ID de la sustancia en PubChem:
24895698
NACRES:
NA.85

origen biológico

Streptomyces hygroscopicus

Nivel de calidad

200

esterilidad

γ-irradiated

concentración

≥60% (HPLC)
45-60 mg/mL in H2O

color

yellow-brown to orange

espectro de actividad antibiótica

viruses

Modo de acción

protein synthesis | interferes

temp. de almacenamiento

2-8°C

cadena SMILES

CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3OC4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O

InChI

1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1

Clave InChI

GRRNUXAQVGOGFE-XKIAHZFYSA-N

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Descripción general

Chemical structure: aminoglycoside

Aplicación

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreading, as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation, and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/mL, specifically at 100 μg/mL for prokaryotes, 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes.

Acciones bioquímicas o fisiológicas

Mode of action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.

Precaución

Hygromycin B products should be stored as supplied at 2-8°C, and the dry solid is stable for at least 5 years if stored at 2-8°C. It is stable at 37°C for 30 days.

Nota de preparación

This product is a solution that is =60% (HPAE), 45-60 mg/mL in H2O, and γ-irradiated .

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Eye Dam. 1 - Resp. Sens. 1

Código de clase de almacenamiento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificados de análisis (COA)

Lot/Batch Number
0000191418
0000191417
0000191416
0000120848
0000191417

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Xiang Liu et al.
Plant cell reports, 32(1), 77-87 (2012-11-01)
KEY MESSAGE : Efficient Agrobacterium -mediated genetic transformation for investigation of genetic and molecular mechanisms involved in inflorescence architectures in Cornus species. Cornus canadensis is a subshrub species in Cornus, Cornaceae. It has recently become a favored non-model plant species
Masayuki Nakamura et al.
Current microbiology, 65(2), 176-182 (2012-05-16)
Agrobacterium tumefaciens-mediated transformation (AtMT) has become a common technique for DNA transformation of yeast and filamentous fungi. In this study, we first established a protocol of AtMT for the phytopathogenic fungus Colletotrichum sansevieriae. Binary T-DNA vector containing the hygromycin B
Goetz Hensel et al.
BMC plant biology, 12, 171-171 (2012-09-26)
While the genetic transformation of the major cereal crops has become relatively routine, to date only a few reports were published on transgenic triticale, and robust data on T-DNA integration and segregation have not been available in this species. Here
M Cruz Muñoz-Centeno et al.
FEMS yeast research, 12(5), 511-520 (2012-03-16)
Using a yeast two-hybrid screen, we isolated a gene from Schizosaccharomyces pombe, whose product interacts with Mpg1, a GDP-mannose-1-phosphate guanylyltransferase involved in the maintenance of cell wall integrity and glycosylation. We have designated this gene mpg2 based on its similarity
Yuko Konishi et al.
BioTechniques, 53(3), 141-152 (2012-09-12)
Gene targeting in a broad range of human somatic cell lines has been hampered by inefficient homologous recombination. To improve this technology and facilitate its widespread application, it is critical to first have a robust and efficient research system for
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