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H3537

Sigma-Aldrich

HEPES solution

99.5%, liquid, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

Sinónimos:

N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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100 ML
$76.20
500 ML
$231.00
1 L
$345.10

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100 ML
$76.20
500 ML
$231.00
1 L
$345.10

About This Item

Número de CAS:
7365-45-9
MDL number:
MFCD00006158
UNSPSC Code:
12161700
PubChem Substance ID:
329815199
NACRES:
NA.25

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Nombre del producto

HEPES solution, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

grade

BioPerformance Certified

Quality Level

200

sterility

0.2 μm filtered

form

liquid

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

Bioburden, tested
 DNase, RNase, Protease, Nickase, free
 endotoxin, tested

pH

5.0-6.0

useful pH range

6.8-8.2

cation traces

Fe: <5 ppm
heavy metals (as Pb): <5 ppm

suitability

suitable for molecular biology

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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General description

HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2]

Application

RNAse HEPES has been used:
  • To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets[3]
  • To recover purified ribonucleotide[4]
  • As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing[5]
  • As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets[6]

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000394584
0000394584
0000394585
0000394586
SLCR5432

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Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes.
Sato M, et al.
Theriogenology, 108, 29-38 (2018)
A Small RNA Isolation and Sequencing Protocol and Its Application to Assay CRISPR RNA Biogenesis in Bacteria.
Silas S, et al.
Bio-protocol, 8(4) (2018)
Masahiro Sato et al.
Theriogenology, 108, 29-38 (2017-12-02)
Recently, successful one-step genome editing by microinjection of CRISPR/Cas9-related mRNA components into the porcine zygote has been described. Given the relatively long gestational period and the high cost of housing swine, the establishment of an effective microinjection-based porcine genome editing
Masahiro Sato et al.
International journal of molecular sciences, 16(8), 17838-17856 (2015-08-08)
Some reports demonstrated successful genome editing in pigs by one-step zygote microinjection of mRNA of CRISPR/Cas9-related components. Given the relatively long gestation periods and the high cost of housing, the establishment of a single blastocyst-based assay for rapid optimization of
Expression of an uncleavable N-terminal RasGAP fragment in insulin-secreting cells increases their resistance toward apoptotic stimuli without affecting their glucose-induced insulin secretion.
Yang JY, et al.
The Journal of Biological Chemistry, 280(38), 32835-32842 (2005)
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