This product is NOT tested for injection into animals. The product is NOT sterile or tested for endotoxin levels.
H3506
Hialuronidasa from bovine testes
Type I-S, lyophilized powder, 400-1000 units/mg solid
Sinónimos:
Hialuronato 4-glucanohidrolasa, Hialuronoglucosaminidasa
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Seleccione un Tamaño
100 MG
$74.70
500 MG
$161.00
1 G
$257.00
5 G
$830.00
Seleccione un Tamaño
Cambiar Vistas
100 MG
$74.70
500 MG
$161.00
1 G
$257.00
5 G
$830.00
About This Item
Número de CAS:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Productos recomendados
type
Type I-S
Quality Level
form
lyophilized powder
specific activity
400-1000 units/mg solid
mol wt
~55 kDa (four subunits of 14 kDa each)
technique(s)
cell culture | mammalian: suitable
single cell analysis: suitable
application(s)
diagnostic assay manufacturing
shipped in
wet ice
storage temp.
−20°C
¿Está buscando productos similares? Visita Guía de comparación de productos
Application
Biochem/physiol Actions
Estas enzimas rompen aleatoriamente los enlaces glucosídicos β-N-acetilhexosamina [1→4] del ácido hialurónico, la condroitina y los sulfatos de condroitina.
La hialuronidasa degrada el hialuronano y se ha observado que su regulación no es apropiada durante la progresión del cáncer.
Unit Definition
Una unidad degradará 0,75 μg del polisacárido ácido hialurónico por minuto a pH 5,35, a 37 ºC (medido por absorción turbidimétrica (λ: 600 nm) cuando forma complejo con la BSA después de 45 minutos).
Other Notes
Véase más información sobre enzimas para análisis de carbohidratos complejos en www.sigma-aldrich.com/enzymeexplorer
Optional
Referencia del producto
Descripción
Precios
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Highly efficient gene transfer into adult ventricular myocytes by recombinant adenovirus.
Kirshenbaum LA et al.
The Journal of Clinical Investigation, 92, 381-381 (1993)
Heikki J Nieminen et al.
Scientific reports, 7(1), 3991-3991 (2017-06-24)
Research is ongoing to develop drug therapies to manage osteoarthritis (OA) and articular cartilage (AC) injuries. However, means to deliver drug to localized AC lesions are highly limited and not clinically available. This study investigates the capability of laser ultrasound
Effect of pressure at primary drying of freeze-drying mouse sperm reproduction ability and preservation potential.
Kawase Y et al.
Reproduction (Cambridge, England), 133, 841-841 (2007)
Moshe Sade-Feldman et al.
Nature communications, 8(1), 1136-1136 (2017-10-27)
Treatment with immune checkpoint blockade (CPB) therapies often leads to prolonged responses in patients with metastatic melanoma, but the common mechanisms of primary and acquired resistance to these agents remain incompletely characterized and have yet to be validated in large
A SAGE (serial analysis of gene expression) view of breast tumor progression.
Porter DA et al.
Cancer Research, 61, 5697-5697 (2001)
Artículos
Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
Protocolos
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
-
Can this be used in animal models in vivo?
1 answer-
Helpful?
-
-
Is there any information available regarding whether hyaluronidase impacts body fluid crystals?
1 answer-
Hyaluronidase is not expected to impact crystals. It is specific to hyaluronan or Hyaluronic acid and may be used with breast and possibly other tissues. However, crystals are typically inorganic salts, with body fluid crystals typically being pyrophosphate. Therefore, hyaluronidase should have no effect upon pyrophosphate crystals.
Helpful?
-
-
Is it acceptable to use H3506 SLCH0779 directly in synovial fluid without dilution?
1 answer-
It is generally not a standard practice to simply sprinkle hyaluronidase (such as H3506 SLCH0779) directly into synovial fluid. For a more formal and generally recognized method, a 0.05% solution in phosphate buffer is recommended, with one drop added per milliliter of fluid. This allows for the clarification of synovial fluids to make cell counts possible. Therefore, directly using H3506 SLCH0779 in synovial fluid without dilution may not be the most common practice.
Helpful?
-
-
What does the type refer to i.e. type I-S?
1 answer-
The type designations for the hyaluronidase enzyme products offered are in-house designations and do not represent any kind of biochemical significance. The types can refer to the order of addition of the product to the catalog, source, physical form, activity, tests performed, etc.
Helpful?
-
-
Can I dissolve H3506 in DMEM/F12 media or PBS before use for tissue dissociation?
1 answer-
It is recommended to dissolve this product at 1 mg/mL in 0.02 M phosphate buffer, pH 7, containing 77 mM sodium chloride and 0.01% BSA. Solubilizing directly into the medium has not been tested, but may be suitable. It is important to include BSA to ensure stability.
See the link below for the recommended instructions:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/296/016/h3506-pis-ms.pdfHelpful?
-
-
I am looking for specific inhibitor for extrasynaptic GABA Rs. The one I found in Sugma is Gaboxadol hydrochloride for ɣ containing containing. Which is the best inhibitor do you have. Is also any extrasynaptic GABA inhibitors for ALPHA subunits GABA?
1 answer-
Searching via the Gene Lookup tool on the main webpage search bar can provide a product list of various related products for any gene product, including agonists, antagonists, antibodies, proteins, RNAi and related gene editing products. Go to the main search bar, select "Genes" from the left dropdown and enter the gene name, gene symbol or gene id to pull a list of gene candidates. Next, select the appropriate gene by clicking on it - the related products will display. For example here is the list of gene products for the GABA A receptor alpha one (GABRA1) https://www.sigmaaldrich.com/US/en/genes/gabra1
Helpful?
-
-
What is the shelf life of Product H3506, Hyaluronidase from bovine testes?
1 answer-
Product H3506, Hyaluronidase from bovine testes, has a Recommended Retest period of two years (period of time between the Quality Control Release and Recommended Retest dates). This date assigns the time frame during which the batch is expected to remain within established specifications if stored under Sigma-Aldrich’s defined conditions, which for product H3506 is at -20°C. This information is provided on the Product's lot specific Certificate of Analysis.
Helpful?
-
-
How is a solution of Hyaluronidase from bovine testes, Product H3506, stored?
1 answer-
Prepare fresh solutions directly before use since stock solutions are not stable for storage.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
Can Hyaluronidase from bovine testes, Product H3506, be used on humans?
1 answer-
No. None of our packaged research products are for human use.
Helpful?
-
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico
