The lyophilized powder may be reconstituted at 5 mg/mL in 0.02M sodium phoshate buffer or 0.45% sodium chloride containing 0.01% BSA. Solutions prepared at concentrations of at least 1 mg/ml may be stored in aliquots at -20°C for several months.
H2126
Hyaluronidase from sheep testes
Type II, lyophilized powder, ≥300 units/mg solid
Sinónimos:
Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-464-3
MDL number:
Specific activity:
≥300 units/mg solid
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Servicio técnico
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Permítanos ayudarleconjugate
conjugate (Glucosaminoglycan)
Quality Segment
type
Type II
form
lyophilized powder
specific activity
≥300 units/mg solid
mol wt
55 kDa
storage temp.
−20°C
General description
Hyaluronidase is a hyaluronic acid-metabolizing enzyme. It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery.
Application
Hyaluronidase from sheep testes has been used:
- in flow cytometry
- in the isolation of putative spermatogonial stem cells (SSCs)
- to study the effects of magnetic stimulation in magnetic hydrogel properties
- in immunohistochemistry to visualize collagen type II distribution
- to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings
- for the degeneration of hydrogels
Biochem/physiol Actions
These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
Physical form
Lyophilized powder containing lactose
Other Notes
One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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Este artículo | ||
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| specific activity ≥300 units/mg solid | specific activity ≥1,500 units/mg solid | specific activity - |
| form lyophilized powder | form lyophilized powder | form film (Clear) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 55 kDa | mol wt 55 kDa | mol wt - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 |
| conjugate conjugate (Glucosaminoglycan) | conjugate - | conjugate conjugate (Glucosaminoglycan) |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Kann die Hyaluronidase H2126 in PBS gelöst werden um sie dann in kleinen Aliquoten bei -20 C zu lagern?
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H2126-100MG Hyaluronidase from sheep testes についてですが、溶解方法を教えてください。
1 answer-
This enzyme is soluble at 1 mg/mL in 20 mM sodium phosphate buffer (pH 7.0), with 77 mM sodium chloride and 0.1 mg/mL BSA at 1 mg/mL. For assay purposes, this product is prepared at 0.3 mg/mL solution in Phosphate Buffer. Heating may be required, Please see the link below to review the full assay protocol:
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/enzymatic-assay-of-hyaluronidase#preparationPlease see the link below to review the product datasheet for a bovine Hyaluronidase, product H3884. This information may also be applied to the ovine source material:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/739/183/h3884pis-ms.pdfHelpful?
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