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GW20071F

Sigma-Aldrich

Anti-β-Galactosidase antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti-Beta-Galactosidase

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About This Item

MDL number:
MFCD00162155
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

chicken

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

bacteria

manufacturer/tradename

Genway 15-288-20071F

technique(s)

western blot: suitable

NCBI accession no.

NP_752394.1

UniProt accession no.

Q8FKG6

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Immunogen

Immunogen Sequence: GI # 26246355, sequence 1-1024
Recombinant β-galactosidase [Escherichia coli CFT073]

Application

Anti-β-Galactosidase antibody produced in chicken is suitable for western blotting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200.

Biochem/physiol Actions

The lac operon (lactose operon) is an essential operon used for the transport and metabolism of lactose in Escherichia coli and some enteric bacteria. It has three adjacent structural genes, lacZ, lacY and lacA. The lacZ gene encodes β-galactosidase. Escherichia coli β-galactosidase is mostly used as a reporter enzyme. It is produced under oxidizing conditions in the cytoplasm of an Origami strain. β-galactosidase occurs in a disulfide bond. At the active site of β-galactosidase a loop opens and closes during catalysis.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Joaquin Seras-Franzoso et al.
Applied and environmental microbiology, 78(7), 2376-2385 (2012-01-31)
Escherichia coli β-galactosidase is probably the most widely used reporter enzyme in molecular biology, cell biology, and biotechnology because of the easy detection of its activity. Its large size and tetrameric structure make this bacterial protein an interesting model for
Rebecca E Harvey et al.
Endocrinology, 158(7), 2179-2189 (2017-04-04)
Ghrelin plays a key role in appetite, energy homeostasis, and glucose regulation. Recent evidence suggests ghrelin suppresses inflammation in obesity; however, whether this is modulated by the acylated and/or des-acylated peptide is unclear. We used mice deficient in acylated ghrelin
Larisa J Jancewicz et al.
Archives of biochemistry and biophysics, 517(2), 111-122 (2011-12-14)
A loop (residues 794-803) at the active site of β-galactosidase (Escherichia coli) opens and closes during catalysis. The α and β carbons of Ser-796 form a hydrophobic connection to Phe-601 when the loop is closed while a connection via two

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