GE17-5268-02

Ni Sepharose^™ High Performance

Cytiva 17-5268-02, pack of 100 mL

• Sinónimos: High Performance Ni Resin, Ni Sepharose
• UNSPSC Code: 41106500
• NACRES: NA.56

Propiedades

• packaging: pack of 100 mL
• manufacturer/tradename: Cytiva 17-5268-02
• matrix: highly cross-linked 6% agarose
• avg. part. size: 34 μm
• cleaning in place: 2-14(Ni²⁺-stripped medium.)
• working range: 3-12(Ni²⁺-stripped medium.)
• capacity: ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

Descripción

General description

Ni Sepharose^™ High Performance affinity media for high-resolution, high capacity purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose^™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni²⁺, which will selectively retain proteins with exposed histidine groups.

The high stability and broad compatibility of Ni Sepharose^™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.

Features and Benefits

• High-performance purification of histidine-tagged proteins
• Negligible leakage of Ni²⁺ ions.
• High binding capacity, at least 40 mg/mL medium
• Compatible with a wide range of reducing agents, detergents, denaturants and other additives
• 34 μm bead size for improved resolution

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.

Sepharose is a trademark of Cytiva

Información de seguridad

• pictograms: GHS09
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids