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GE17-5179-01

Sigma-Aldrich

Mono Q® 4.6/100 PE

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About This Item

Código UNSPSC:
41115700
NACRES:
SB.52

ligand

-OCH2CH(OH)CH2OCH2CH(OH)CH2N+(CH3)3

Materiales

PEEK column

descripción

Ion Exchanger Type (value)

envase

pkg of 1 ea

Parámetros

580 psi

lecho volumen

1.7 mL

columna D.I.

4.6 mm

Matriz

monodisperse porous polystyrene/divinyl benzene beads
polymeric

grupo activo de la matriz

ammonium ion, quaternary phase

tam. prom. pieza

10 μm

cleaning

1-14

Intervalo de trabajo

2-12

técnica de separación

anion exchange

Descripción general

Mono Q® is a strong anion exchanger prepacked with MonoBeads in a Tricorncolumn. The column is an excellent choice for small-scale polishing in purification of proteins, peptides, and other biomolecules when high purity is required.

The Tricorn columns offer high-resolution separations with greater loading capacity but slightly lower resolution than MiniBeads columns.

Características y beneficios

  • Monodisperse 10 μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
  • Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q ion exchangers.
  • Compatible with AKTA design, FPLC™ System, and other high-performance LC systems.
  • MonoBeads are also available as Mono P® for chromatofocusing

Almacenamiento y estabilidad

Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Información legal

FPLC is a trademark of Cytiva
Mono P is a registered trademark of Cytiva
Mono Q is a registered trademark of Cytiva

Pictogramas

Flame
GHS02

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

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Certificados de análisis (COA)

Lot/Batch Number

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Artículos

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocolos

MonoBeads: Purification of Milligram Quantities with Highest Resolution

This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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