To reconstitute dissolve G6378, Glucose-6-phosphate Dehydrogenase, in water and a dilute buffer. Following reconstitution, it should be refrigerated at 4°C, with stock solutions remaining stable for up to 6 months at this temperature.
G6378
Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)
Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)
Sinónimos:
G-6-P-DH, Zwischenferment
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100 UNITS
$66.40
250 UNITS
$122.00
500 UNITS
$216.00
1000 UNITS
$427.00
2000 UNITS
$731.00
5000 UNITS
$1,490.00
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100 UNITS
$66.40
250 UNITS
$122.00
500 UNITS
$216.00
1000 UNITS
$427.00
2000 UNITS
$731.00
5000 UNITS
$1,490.00
About This Item
Productos recomendados
tpo
Type XV
Nivel de calidad
Formulario
lyophilized powder
actividad específica
200-400 units/mg protein (modified Warburg-Christian)
mol peso
128 kDa
purificado por
crystallization
aplicaciones
diagnostic assay manufacturing
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
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Descripción general
Aplicación
Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm.[3]
Acciones bioquímicas o fisiológicas
Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway<<<17>>>.
Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Definición de unidad
One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.
Forma física
Lyophilized and essentially sulfate-free; contains approx. 20% sodium citrate based on dry weight
Nota de preparación
Prepared from G7877
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Certificados de análisis (COA)
Lot/Batch Number
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Yong Yang et al.
Frontiers in microbiology, 9, 1428-1428 (2018-07-20)
Mycobacteria spontaneously form surface-associated multicellular communities, called biofilms, which display resistance to a wide range of exogenous stresses. A causal relationship between biofilm formation and emergence of stress resistance is not known. Here, we report that activation of a nitrogen
D C Doehlert
Plant physiology, 84(3), 830-834 (1987-07-01)
Ketose reductase (NAD-dependent polyol dehydrogenase EC 1.1.1.14) activity, which catalyzes the NADH-dependent reduction of fructose to sorbitol (d-glucitol), was detected in developing maize (Zea mays L.) endosperm, purified 104-fold from this tissue, and partially characterized. Product analysis by high performance
K E Reilly et al.
Biochemical and biophysical research communications, 216(3), 993-998 (1995-11-22)
A commercial preparation of glucose-6-phosphate dehydrogenase (G6PD) purified from Saccharomyces cerevisiae was subjected to PAGE analysis under both nondenaturing and denaturing conditions. The enzyme, identified by both activity staining and anti-yeast G6PD antibody immunoblotting, was shown to contain carbohydrate using
P Andrews
The Biochemical journal, 96(3), 595-606 (1965-09-01)
1. Correlation between elution volume, V(e), and molecular weight was investigated for gel filtration of proteins of molecular weights ranging from 3500 (glucagon) to 820000 (alpha-crystallin) on Sephadex G-200 columns at pH7.5. 2. Allowing for uncertainties in the molecular weights
Anna Leonov et al.
Oncotarget, 13, 918-943 (2022-08-09)
We propose a hypothesis of a mechanism linking cellular aging to cellular quiescence in chronologically aging budding yeast. Our hypothesis posits that this mechanism integrates four different processes, all of which are initiated after yeast cells cultured in a medium
Protocolos
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
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What are the recommended reconstitution and storage procedures for item G6378, Glucose-6-phosphate Dehydrogenase from baker's yeast (S. cerevisiae)?
1 answer-
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What is the activity and protein content of the lot number of Product G6378, Glucose-6-phosphate Dehydrogenase, I have?
1 answer-
The protein content and the specific activity of the enzyme can be found on the lot-specific certificate of analysis.
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What does Type XV mean in the description of Product G6378, Glucose-6-phosphate Dehydrogenase?
1 answer-
The type designation Type XV is a Sigma in-house designation and does not represent any kind of biochemical significance. It simply refers to the order of addition of the product to the catalog (i.e., first there was a type I, then a type II, etc.).
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What are the Km values for Product G6378, Glucose-6-phosphate Dehydrogenase?
1 answer-
The Km values for glucose 6-phosphate and NADP are 2.0 x 10-5 and 2.0 x 10-6 , respectively in Tris buffer, pH 8.0 containing 10 mm magnesium chloride at 38°C.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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