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G5768

Sigma-Aldrich

Gamborg′s B-5 Basal Salt Mixture

fine powder, suitable for plant cell culture

Sinónimos:

B-5 Salt Mixture, Basal Salt Mixture, Gamborg B-5 Medium

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.72
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Formulario

fine powder

Nivel de calidad

técnicas

cell culture | plant: suitable

aplicaciones

agriculture

temp. de almacenamiento

2-8°C

Descripción general

Gamborg′ B-5 medium described by Gamborg, et al. [1] may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro plant culture of cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis.

Aplicación

Gamborg′s B-5 Basal Salt Mixture has been used:
  • as a constituent during the preparation of TEX buffer[2]
  • as a media to maintain potato suspension and callus cultures[3]
  • as a media in the primary-node transformation method[4]

Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.

Variante de la fórmula

With the concentrations of macro- and micronutrients as described by Gamborg, et al. (1968).

Media Formulation

Cantidad

Formulated to contain 3.1 grams of powder per liter of medium.

Pictogramas

Flame over circle

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Ox. Sol. 3

Código de clase de almacenamiento

5.1B - Oxidizing hazardous materials

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Vinod Vijayakumar et al.
Plant, cell & environment, 39(2), 393-415 (2015-08-25)
Interaction of plant roots with arbuscular mycorrhizal fungi (AMF) is a complex trait resulting in cooperative interactions among the two symbionts including bidirectional exchange of resources. To study arbuscular mycorrhizal symbiosis (AMS) trait variation in the model plant Lotus japonicus
Differential induction of redox sensitive extracellular phenolic amides in potato
Baker CJ, et al.
Physiological and Molecular Plant Pathology, 73(4-5), 109-115 (2008)
A novel Agrobacterium rhizogenes-mediated transformation method of soybean [Glycine max (L.) Merrill] using primary-node explants from seedlings
Olhoft PM, et al.
In Vitro Cellular & Developmental Biology. Plant, 43(6), 536-549 (2007)
Isolation of Intact Vacuoles from Petunia Petals and Extraction of Sequestered Glycosylated Phenylpropanoid Compounds
Skaliter O, et al.
Bio-protocol, 8(13) (2018)
Li-Da Wang et al.
Journal of Asian natural products research, 4(3), 171-174 (2002-07-18)
Callus cultures of Annona squamosa were induced using different explants including petals, seed contents (megagametophyte and embryo) and fruits (mesocarp). Growth of the calli induced from the explants was found to be influenced by the type, concentration and ratio of

Artículos

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.

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