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MilliporeSigma

G4423

Sigma-Aldrich

β-Glucanase from Trichoderma longibrachiatum

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Sinónimos:

endo-1,3(4)-β-glucanase, endoglucanase

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100 G
$184.00

$184.00


Fecha estimada de envío08 de abril de 2025DesdeMILWAUKEE



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100 G
$184.00

About This Item

Comisión internacional de enzimas:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

$184.00


Fecha estimada de envío08 de abril de 2025DesdeMILWAUKEE


Formulario

powder

Nivel de calidad

actividad específica

≥1.0 units/mg solid

composición

protein, ≥10%

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Descripción general

β -Glucanase represents a group of carbohydrate enzymes which break down glycosidic bonds within beta-glucan.[1] It forms the main constituent of fungal cell walls and could be a potential structural and storage polysaccharide of marine macro-algae. It has the ability to degrade fungal cell walls and may be involved in defense mechanism of plants against pathogenic fungi.[1]
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles.

Aplicación

β -Glucanase was used as a cellulase enzyme in the combined biological and chemical pretreatment method for lignocellulosic ethanol production from energy cane. It was also used in the enzymatic saccharification of cellulose and production of ethanol. [2]

Acciones bioquímicas o fisiológicas

A mixture composed mainly of β-1→3 / 1→4-glucanase, xylanase, and cellulase activities. β-glucosidase, β-xylosidase, α-L-arabinofuranosidase, amylase, and protease activities are also present.[3][4]

Definición de unidad

One unit will liberate 1.0 μmole of glucose from cellulose in one hr at pH 5.0 at 37 °C (2 hr incubation time).

Forma física

contains maltodextrin, silica and sodium benzoate

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Certificados de análisis (COA)

Lot/Batch Number

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Combined biological and chemical pretreatment method for lignocellulosic ethanol production from energy cane;
Sara Shields, Raj Boopathy
Renewable Bioresources null
David J Adams
Microbiology (Reading, England), 150(Pt 7), 2029-2035 (2004-07-17)
The fungal cell wall is a complex structure composed of chitin, glucans and other polymers, and there is evidence of extensive cross-linking between these components. The wall structure is highly dynamic, changing constantly during cell division, growth and morphogenesis. Hydrolytic
Ravindra Bansal et al.
Current genetics, 65(4), 953-964 (2019-03-07)
Trichoderma spp. are widely used as commercial biofungicides, and most commercial formulations are conidia based. Identification of genes that regulate conidiation would thus be of help in genetic reprogramming of these species to optimize sporulation. In this study, we constructed
Marie-Therese Eckstein et al.
Current biology : CB, 30(23), 4799-4807 (2020-10-10)
The human gut microbiota is composed of diverse microbes that not only compete but also rely on one another for resources and access to microhabitats in the intestine [1, 2]. Indeed, recent efforts to map the microbial biogeography of the
Nhuan P Nghiem et al.
Applied biochemistry and biotechnology, 165(3-4), 870-882 (2011-06-15)
A fermentation process, which was designated the enhanced dry grind enzymatic (EDGE) process, has recently been developed for barley ethanol production. In the EDGE process, in addition to the enzymes normally required for starch hydrolysis, commercial β-glucanases were used to

Questions

1–3 of 3 Questions  
  1. How much protease activity is present?

    1 answer
    1. The minimum activity of this ?-glucanase is 1 unit per milligram. The activity varies from lot to lot and is reported in the Certificate of Analysis. This product has not been tested for any additional protease activity. Please see the link below to review a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/g4423#product-documentation

      Helpful?

  2. Is this product suitable for use in food preparation?

    1 answer
    1. No. Unless otherwise stated, this product is intended for research use only. It is not suitable for any other purpose, which includes, but is not limited to, unauthorized commercial use, in vitro diagnostic use, or therapeutic use in humans or animals.

      Helpful?

  3. Hi, I am planning to use β-Glucanase G4423 to digest the Pyhtium inosidieus cell wall to let the probes for In situ hybridozation . May I know the conditions, concentration and dilution buffer for this product? Thanks Best

    1 answer
    1. This product has not been testd in In Situ Hybridization in house. Below is a link to an article citing this product in ISH.

      https://www.nature.com/articles/s42003-021-02693-y

      Helpful?

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